Vasincu Alexandru, Paulsen Berit S, Diallo Drissa, Vasincu Ioana, Aprotosoaie Ana C, Bild Veronica, Charalambous Christiana, Constantinou Andreas I, Miron Anca, Gavrilescu Cristina M
Department of Pharmaceutical Sciences II, Faculty of Pharmacy, University of Medicine and Pharmacy Grigore T. Popa-Iasi, Universitatii Str. 16, 700115 Iasi, Romania.
Section of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, N-0316 Oslo, Norway.
Molecules. 2014 Nov 19;19(11):19114-36. doi: 10.3390/molecules191119114.
The roots of Vernonia kotschyana Sch. Bip. ex Walp. (Asteraceae) are used in Malian traditional medicine in the treatment of gastroduodenal ulcers and gastritis. Since oxidative stress is involved in gastric ulceration, the aim of this study was to screen the root extracts for their in vitro antioxidant activity and phenolic content. The roots were extracted successively with chloroform, ethyl acetate, ethanol and water. The antioxidant activity of root extracts was evaluated in both cell-free and cell-based assays. Their chemical characterization was performed by Fourier transform infrared spectroscopy (FT-IR) whereas the total phenolic content was determined by the Folin-Ciocalteu method. The ethyl acetate extract displayed the highest phenolic content and was found to be the most active in the free radical scavenging and lipid peroxidation inhibition assays; it also showed a high antioxidant activity in MCF-12F cells. This study suggests a potential use of the ethyl acetate extract of Vernonia kotschyana not only as an antioxidant agent in gastroduodenal ulcers and gastritis, but also in other disorders characterized by high levels of oxidative stress.
科氏斑鸠菊（Vernonia kotschyana Sch. Bip. ex Walp.，菊科）的根在马里传统医学中用于治疗胃十二指肠溃疡和胃炎。由于氧化应激与胃溃疡有关，本研究旨在筛选其根提取物的体外抗氧化活性和酚类含量。依次用氯仿、乙酸乙酯、乙醇和水对根进行提取。在无细胞和细胞试验中评估根提取物的抗氧化活性。通过傅里叶变换红外光谱（FT-IR）对其进行化学表征，而总酚含量则采用福林-西奥尔特法测定。乙酸乙酯提取物的酚类含量最高，在自由基清除和脂质过氧化抑制试验中活性最强；在MCF-12F细胞中也显示出高抗氧化活性。本研究表明，科氏斑鸠菊乙酸乙酯提取物不仅有可能作为胃十二指肠溃疡和胃炎的抗氧化剂，还可用于其他以氧化应激水平高为特征的疾病。
