Department of Oral and Maxillofacial Surgery, Dental School, University of Athens, Athens, Greece.
Implant Dent. 2012 Oct;21(5):399-405. doi: 10.1097/ID.0b013e3182611f48.
To evaluate the effect of autologous platelet-rich plasma (PRP) on the early phases of osteoinduction by allogenic demineralized bone matrix (DBM) in rabbit intramuscular positions.
Allogenic DBM was produced from bones of 3 healthy rabbits. In each of 6 experimental animals, 0.3 mL autologous PRP was prepared and 2 muscle pouches were created, where 250 mg DBM + PRP (experimental sites) and 250 mg DBM without PRP (control sites) were randomly implanted. Animals were euthanized 3 weeks postoperatively.
Histologic examination revealed uneventful healing in all cases, whereas remineralization of the periphery of the bone graft particles was a constant finding. In both control and experimental sites, fibroblasts and other mesenchymal cells (probably osteoprogenitor cells and preosteoblasts) were observed. The main histological difference was the recolonization of the empty lacunae of the bone graft particles by osteocytes at the control sites. The degradation of the graft at the control sites was statistically significantly quicker, although a statistically significant difference regarding the amount of the newly formed fibrous connective tissue was not observed.
The present study demonstrated that in this experimental model, the addition of PRP to DBM had a negative effect on the early phases of osteoinduction at 3 weeks of observation.
评估自体富血小板血浆（PRP）对同种脱矿骨基质（DBM）在兔肌肉内诱导成骨早期阶段的影响。
同种异体 DBM 由 3 只健康兔的骨骼制备。在 6 只实验动物中，每只动物制备 0.3 毫升自体 PRP，并创建 2 个肌袋，其中随机植入 250mg DBM+PRP（实验组）和 250mg DBM 无 PRP（对照组）。术后 3 周处死动物。
所有病例均无并发症愈合，而骨移植物颗粒周围的再矿化是一个恒定的发现。在对照组和实验组中，均观察到成纤维细胞和其他间充质细胞（可能为骨祖细胞和前成骨细胞）。主要的组织学差异是对照组骨移植物颗粒的空陷窝被成骨细胞重新占据。尽管在新形成的纤维结缔组织数量方面未观察到统计学差异，但对照组移植物的降解速度明显更快。
本研究表明，在该实验模型中，PRP 与 DBM 联合应用对观察 3 周时的成骨早期阶段有负面影响。
