Nagata Maria J H, Melo Luiz G N, Messora Michel R, Bomfim Suely R M, Fucini Stephen E, Garcia Valdir G, Bosco Alvaro F, Okamoto Tetuo
Department of Surgery and Integrated Clinic, Division of Periodontics, Dental School of Araçatuba, São Paulo State University - UNESP, São Paulo, Brazil.
J Clin Periodontol. 2009 Sep;36(9):775-83. doi: 10.1111/j.1600-051X.2009.01450.x. Epub 2009 Jul 14.
This study histologically analysed the effect of autogenous platelet-rich plasma (PRP), prepared according to a new semiautomatic system, on healing of autogenous bone (AB) grafts placed in surgically created critical-size defects (CSD) in rabbit calvaria.
Sixty rabbits were divided into three groups: C, AB and AB/PRP. A CSD was created in the calvarium of each animal. In Group C (control), the defect was filled by blood clot only. In Group AB (autogenous bone graft), the defect was filled with particulate autogenous bone. In Group AB/PRP (autogenous bone graft with platelet-rich plasma), it was filled with particulate autogenous bone combined with PRP. All groups were divided into subgroups (n=10) and euthanized at 4 or 12 weeks post-operatively. Histometric and histologic analyses were performed. Data were statistically analysed (anova, t-test, p<0.05).
Group C presented significantly less bone formation compared with Group AB and AB/PRP in both periods of analysis (p<0.001). At 4 weeks, Group AB/PRP showed a statistically greater amount of bone formation than Group AB (64.44 +/- 15.0%versus 46.88 +/- 14.15%; p=0.0181). At 12 weeks, no statistically significant differences were observed between Groups AB and AB/PRP (75.0 +/- 8.11%versus 77.90 +/- 8.13%; p>0.05). It is notable that the amount of new bone formation in Group AB/PRP at 4 weeks was similar to that of Group AB at 12 weeks (p>0.05).
Within its limitation, the present study has indicated that (i) AB and AB/PRP significantly improved bone formation and (ii) a beneficial effect of PRP was limited to an initial healing period of 4 weeks.
本研究通过组织学分析，探讨根据一种新型半自动系统制备的自体富血小板血浆（PRP）对植入兔颅骨手术造成的临界尺寸缺损（CSD）处自体骨（AB）移植物愈合的影响。
60只兔子分为三组：C组、AB组和AB/PRP组。在每只动物的颅骨上制造一个CSD。C组（对照组）仅用血凝块填充缺损；AB组（自体骨移植组）用颗粒状自体骨填充缺损；AB/PRP组（自体骨移植加富血小板血浆组）用颗粒状自体骨与PRP联合填充缺损。所有组再分为亚组（n = 10），并在术后4周或12周实施安乐死。进行组织计量学和组织学分析。对数据进行统计学分析（方差分析、t检验，p < 0.05）。
在两个分析时间段内，C组的骨形成均显著少于AB组和AB/PRP组（p < 0.001）。在4周时，AB/PRP组的骨形成量在统计学上高于AB组（64.44±15.0%对46.88±14.15%；p = 0.0181）。在12周时，AB组和AB/PRP组之间未观察到统计学上的显著差异（75.0±8.11%对77.90±8.13%；p > 0.05）。值得注意的是，AB/PRP组在4周时的新骨形成量与AB组在12周时的相似（p > 0.05）。
在其局限性范围内，本研究表明：（i）AB和AB/PRP显著改善了骨形成；（ii）PRP的有益作用仅限于最初4周的愈合期。
