Kinoshita Emiko, Hayashi Kyoko, Katayama Hiroshi, Hayashi Toshimitsu, Obata Akio
Research and Development Division, Kikkoman Corporation, 399 Noda, Noda, Chiba 278-0037, Japan.
Biosci Biotechnol Biochem. 2012;76(9):1633-8. doi: 10.1271/bbb.120112. Epub 2012 Sep 7.
Elderberry (Sambucus nigra L.) has traditionally been used for treating influenza and colds. We evaluated the antiviral effect of concentrated juice of elderberry (CJ-E) on the human influenza A virus (IFV). CJ-E had a relatively strong effect on IFV-infected mice, although its anti-IFV activity was weak in a cell culture system. The in vivo anti-IFV activities of the fractions were determined after separating CJ-E by ultrafiltration and anion-exchange chromatography. Oral administration of the high-molecular-weight fractions of CJ-E to IFV-infected mice suppressed viral replication in the bronchoalveolar lavage fluids (BALFs), and increased the level of the IFV-specific neutralizing antibody in the serum, as well as the level of secretory IgA in BALFs and feces. Fr. II from high-molecular-weight fraction HM, which contained acidic polysaccharides, showed relatively strong defense against IFV infection. We conclude that CJ-E had a beneficial effect by the stimulating immune response and preventing viral infection.
接骨木（黑接骨木）传统上一直用于治疗流感和感冒。我们评估了接骨木浓缩汁（CJ-E）对人甲型流感病毒（IFV）的抗病毒作用。CJ-E对感染IFV的小鼠有相对较强的作用，尽管其在细胞培养系统中的抗IFV活性较弱。通过超滤和阴离子交换色谱法分离CJ-E后，测定了各组分的体内抗IFV活性。给感染IFV的小鼠口服CJ-E的高分子量组分可抑制支气管肺泡灌洗液（BALF）中的病毒复制，并提高血清中IFV特异性中和抗体的水平，以及BALF和粪便中分泌型IgA的水平。高分子量组分HM中的Fr. II含有酸性多糖，对IFV感染表现出相对较强的防御作用。我们得出结论，CJ-E通过刺激免疫反应和预防病毒感染具有有益作用。
