Department of Internal Medicine-UNESP, Universidade Estadual Paulista, Botucatu, Sao Paulo, Brazil.
J Nephrol. 2013 May-Jun;26(3):534-9. doi: 10.5301/jn.5000200. Epub 2012 Sep 11.
Granulocyte colony-stimulating factor (G-CSF) and Erythropoietin (EPO) are known to stimulate the growth and differentiation of progenitor cells to prevent acute renal injury. This study aimed to assess the use of growth factors to mobilize stem cell in a mouse model of adriamycin-induced chronic kidney disease.
All animals were injected with adriamycin for kidney injury and allocated into three treatment groups (G-CSF, EPO and G-CSF + EPO), and a control group (adriamycin alone).
Number of atrophic sites, glomerulosclerosis rate and interstitial fibrosis severity score were assessed in all groups. In all treatment groups, histologic parameters did not significantly differ, but were lower than in the control group (P<.001). Scal and CD34 expressions among treatment groups showed no statistically significant difference, but were higher than in the control group (P<.0001). CD105 expression was higher in EPO and G+EPO as compared to G-CSF and the control group (P<.0001), with no statistically significant difference between the latter two groups (P = NS).
G-CSF and EPO had a histologic protective effect, while treatment with EPO + G-CSF had no additive effects in a model of adriamycin-induced chronic kidney disease.
粒细胞集落刺激因子（G-CSF）和促红细胞生成素（EPO）已知可刺激祖细胞的生长和分化，以预防急性肾损伤。本研究旨在评估生长因子在阿霉素诱导的慢性肾病小鼠模型中动员干细胞的作用。
所有动物均注射阿霉素造成肾损伤，并分为三组（G-CSF、EPO 和 G-CSF+EPO）和对照组（仅阿霉素）。
在所有组中评估了萎缩部位的数量、肾小球硬化率和间质纤维化严重程度评分。在所有治疗组中，组织学参数无显著差异，但均低于对照组（P<.001）。各组之间的 Scal 和 CD34 表达没有统计学上的显著差异，但均高于对照组（P<.0001）。与 G-CSF 和对照组相比，EPO 和 G+EPO 中的 CD105 表达更高（P<.0001），后两者之间无统计学差异（P=NS）。
G-CSF 和 EPO 具有组织保护作用，而 EPO+G-CSF 在阿霉素诱导的慢性肾病模型中没有附加作用。
