丙型肝炎病毒包膜糖蛋白 E2 抗原位点 412 至 423 与抗体 AP33 复合物的结构。
Structure of hepatitis C virus envelope glycoprotein E2 antigenic site 412 to 423 in complex with antibody AP33.
机构信息
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, USA.
出版信息
J Virol. 2012 Dec;86(23):13085-8. doi: 10.1128/JVI.01939-12. Epub 2012 Sep 12.
Abstract
We have determined the crystal structure of the broadly neutralizing antibody (bnAb) AP33, bound to a peptide corresponding to hepatitis C virus (HCV) E2 envelope glycoprotein antigenic site 412 to 423. Comparison with bnAb HCV1 bound to the same epitope reveals a different angle of approach to the antigen by bnAb AP33 and slight variation in its β-hairpin conformation of the epitope. These structures establish two different modes of binding to E2 that antibodies adopt to neutralize diverse HCV.
摘要
我们已经确定了广谱中和抗体（bnAb）AP33 与对应丙型肝炎病毒（HCV）E2 包膜糖蛋白抗原表位 412 至 423 的肽段结合的晶体结构。与结合同一表位的 bnAb HCV1 进行比较，揭示了 bnAb AP33 接近抗原的不同角度，以及其表位β发夹构象的轻微变化。这些结构确定了抗体采用的两种不同的结合 E2 的方式，以中和不同的 HCV。
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