Cranfield Health, Cranfield University, Bedfordshire, UK.
J Sex Med. 2012 Nov;9(11):2770-84. doi: 10.1111/j.1743-6109.2012.02916.x. Epub 2012 Sep 13.
Experimental studies investigating physiology of erectile function and pathophysiology erectile dysfunction employ several in vitro and in vivo techniques. As the field of sexual medicine expanding, the proper conduct of such techniques is becoming an even more important necessity than before.
This review article aims to guide scientists, particularly young researchers and new comers in the field, toward employment of these techniques in an appropriate, timely, and competent fashion.
The authors reviewed the existing available published articles on the following topics: intracavernosal pressure measurements, cavernous nerve injury models, nitric oxide-cyclic guanosine monophosphate pathway, hypertension- and smoking-induced erectile dysfunction models, and stem cells.
The authors present a consensus on how to best perform these models and techniques and also highlight the pitfalls.
The authors hope that this article will assist and encourage young scientists in the field and that similar articles covering other important models will be also available to them soon.
研究勃起功能生理学和勃起功能障碍病理生理学的实验研究采用了多种体外和体内技术。随着性医学领域的不断发展,正确实施这些技术比以往任何时候都更加必要。
本文旨在为科学家,特别是年轻研究人员和该领域的新手,提供指导,帮助他们以适当、及时和熟练的方式使用这些技术。
作者回顾了以下主题的现有已发表文章:海绵体内压测量、海绵体神经损伤模型、一氧化氮-环鸟苷单磷酸途径、高血压和吸烟引起的勃起功能障碍模型以及干细胞。
作者就如何最好地进行这些模型和技术提出了共识,并强调了其中的陷阱。
作者希望本文将帮助和鼓励该领域的年轻科学家,并且他们很快也能获得涵盖其他重要模型的类似文章。