Weyne Emmanuel, Ilg Marcus M, Cakir Onur Omer, Muneer Asif, Roussel Delphine Behr, Albersen Maarten, Angulo Javier, Corona Giovanni, Bettocchi Carlo, Reisman Yacov, Castiglione Fabio
Laboratory for Experimental Urology, Organ Systems, Department of Development and Regeneration, University of Leuven, Leuven, Belgium.
Faculty of Health, Education, Medicine and Social Care, Medical Technology Research Centre, Anglia Ruskin University, Chelmsford, UK.
Sex Med. 2020 Sep;8(3):327-337. doi: 10.1016/j.esxm.2020.06.007. Epub 2020 Jul 13.
Rodent animal models are currently the most used in vivo model in translational studies looking into the pathophysiology of erectile dysfunction after nerve-sparing radical prostatectomy.
This European Society for Sexual Medicine (ESSM) statement aims to guide scientists toward utilization of the rodent model in an appropriate, timely, and proficient fashion.
MEDLINE and EMBASE databases were searched for basic science studies, using a rodent animal model, looking into the consequence of pelvic nerve injury on erectile function.
The authors present a consensus on how to best perform experiments with this rodent model, the details of the technique, and highlight possible pitfalls.
Owing to the specific issue-basic science-Oxford 2011 Levels of Evidence criteria cannot be applied. However, ESSM statements on this topic will be provided in which we summarize the ESSM position on various aspects of the model such as the use of the Animal Research Reporting In Vivo Experiments guideline and the of common range parameter for nerve stimulation. We also highlighted the translational limits of the model.
The following statements were formulated as a suggestive guidance for scientists using the cavernous nerve injury model. With this, we hope to standardize and further improve the quality of research in this field. It must be noted that this model has its limitations. Weyne E, Ilg MM, Cakir OO, et al. European Society for Sexual Medicine Consensus Statement on the Use of the Cavernous Nerve Injury Rodent Model to Study Postradical Prostatectomy Erectile Dysfunction. Sex Med 2020;8:327-337.
啮齿动物模型是目前在探索保留神经的根治性前列腺切除术后勃起功能障碍病理生理学的转化研究中使用最广泛的体内模型。
本欧洲性医学学会（ESSM）声明旨在指导科学家以适当、及时和熟练的方式利用啮齿动物模型。
检索MEDLINE和EMBASE数据库，查找使用啮齿动物模型研究盆腔神经损伤对勃起功能影响的基础科学研究。
作者就如何最好地使用该啮齿动物模型进行实验、技术细节达成共识，并强调可能存在的陷阱。
由于具体问题——基础科学——无法应用2011年牛津证据水平标准。然而，将提供ESSM关于该主题的声明，其中我们总结了ESSM在模型各个方面的立场，如动物研究体内实验报告指南的使用以及神经刺激的常见范围参数。我们还强调了该模型的转化局限性。
以下声明是为使用海绵体神经损伤模型的科学家制定的指导性建议。借此，我们希望规范并进一步提高该领域的研究质量。必须指出，该模型有其局限性。韦内E、伊尔格MM、恰基尔OO等。欧洲性医学学会关于使用海绵体神经损伤啮齿动物模型研究根治性前列腺切除术后勃起功能障碍的共识声明。性医学2020；8：327 - 337。
