Universität Würzburg, Institut für Pharmakologie und Toxikologie, Würzburg, Germany.
Mutat Res. 2012 Oct-Nov;738-739:71-4. doi: 10.1016/j.mrfmmm.2012.08.004. Epub 2012 Sep 4.
Elevated temperature can cause biological effects in vitro and in vivo. Many studies on effects of hypo- and hyperthermia have been conducted, but only few studies systematically investigated the formation of genomic damage in the micronucleus test in human cells in vitro as a consequence of different temperatures. In the present study, HaCaT human keratinocytes were exposed to different temperatures from 37°C to 42°C for 24h in a regular cell culture incubator. Micronucleus frequency as a marker of genomic damage was elevated in a temperature-dependent and statistically significant manner. Apoptosis occurred at temperatures of 39°C or higher. Cell proliferation was unaffected up to 40°C and decreased at 41°C and 42°C. Expression of the heat shock protein Hsp70 was elevated, particularly at temperatures of 40°C and higher. These findings are in agreement with several in vivo studies and some in vitro studies looking at single, specific temperatures, but a systematically investigated temperature-dependent increase of genomic damage in human keratinocytes in vitro is demonstrated for the first time here.
高温可在体外和体内引起生物学效应。已经有许多关于低热和高热效应的研究，但只有少数研究系统地调查了不同温度下体外人细胞微核试验中基因组损伤的形成。在本研究中，HaCaT 人角质形成细胞在常规细胞培养孵育箱中暴露于 37°C 至 42°C 的不同温度下 24 小时。微核频率作为基因组损伤的标志物呈温度依赖性和统计学显著升高。在 39°C 或更高温度下发生细胞凋亡。细胞增殖在 40°C 以下不受影响，在 41°C 和 42°C 时减少。热休克蛋白 Hsp70 的表达升高，尤其是在 40°C 及以上温度下。这些发现与一些体内研究和一些研究单一特定温度的体外研究一致，但这里首次证明了体外人角质形成细胞中基因组损伤随温度升高的系统研究。
