Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, UP, India.
Biochimie. 2012 Dec;94(12):2776-82. doi: 10.1016/j.biochi.2012.09.001. Epub 2012 Sep 10.
The acute toxicity of potassium bromate (KBrO(3)) on rat small intestine was studied in this work. Animals were given a single oral dose of KBrO(3) (100 mg/kg body weight) and sacrificed 12, 24, 48, 96 and 168 h after the treatment; control animals were not given KBrO(3). The administration of KBrO(3) resulted in a reversible decline in the specific activities of several BBM enzymes. Lipid peroxidation, protein oxidation and hydrogen peroxide levels increased while total sulfhydryl groups and reduced glutathione decreased in KBrO(3)-treated rats indicating induction of oxidative stress in the intestinal mucosa. The activities of anti-oxidant and carbohydrate metabolic enzymes were also altered upon KBrO(3) treatment. The maximum changes in all the parameters were 48 h after administration of KBrO(3) after which recovery took place, in many cases almost to control values after 168 h. Histopathological studies supported the biochemical findings showing extensive damage to the intestine at 48 h and recovery at 168 h. These results show that a single oral dose of KBrO(3) causes reversible oxidative damage to the intestine.
本研究旨在探讨重铬酸钾（KBrO3）对大鼠小肠的急性毒性。动物经口给予单剂量重铬酸钾（100mg/kg 体重），分别于给药后 12、24、48、96 和 168 小时处死；对照组动物未给予重铬酸钾。KBrO3 处理导致几种 BBM 酶的比活力可逆性下降。脂质过氧化、蛋白质氧化和过氧化氢水平升高，而总巯基和还原型谷胱甘肽减少，表明肠道黏膜发生氧化应激。KBrO3 处理还改变了抗氧化和糖代谢酶的活性。所有参数的最大变化发生在给予 KBrO3 后 48 小时，此后开始恢复，在许多情况下，168 小时后几乎恢复到对照值。组织病理学研究支持生化发现，在 48 小时时观察到肠道广泛损伤，而在 168 小时时恢复。这些结果表明，单次口服给予重铬酸钾可导致肠道可逆性氧化损伤。
