Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe-shi 657-8501 Japan.
Gene. 2012 Dec 1;510(2):133-41. doi: 10.1016/j.gene.2012.08.036. Epub 2012 Sep 11.
We cloned a cDNA encoding inhibitor of apoptosis (IAP) from the greater wax moth, Galleria mellonella. The deduced amino acid sequence showed that GmIAP contains two baculoviral IAP repeat (BIR) motifs, followed by a RING finger. The sequence comparison showed that GmIAP had high homology to lepidopteran IAPs and baculoviral IAPs, as well as dipteran IAPs. GmIAP transcript and its protein appeared in both the midgut and the silk gland during metamorphosis and starvation where cell death was detected by TUNEL test. IAP, and capases-1, -3, -4 and -6 appeared as at least two peaks in the midgut and silk gland during metamorphosis. Caspase-1 transcript appeared at the highest level among caspases, while caspase-3 and caspase-6 seemed to be the most relevant caspases to IAP during metamorphosis suggesting that IAP and caspases may be involved in a core apoptosis pathway in the wax moth as in flies and mosquitoes.
我们从大蜡螟（Galleria mellonella）中克隆了凋亡抑制因子（IAP）的 cDNA。推导的氨基酸序列表明，GmIAP 含有两个杆状病毒 IAP 重复（BIR）基序，后面是一个 RING 指结构。序列比较表明，GmIAP 与鳞翅目昆虫 IAP 和杆状病毒 IAP 以及双翅目昆虫 IAP 具有高度同源性。GmIAP 转录本及其蛋白在变态和饥饿期间均出现在中肠和丝腺中，TUNEL 试验检测到细胞死亡。IAP 以及 caspase-1、-3、-4 和 -6 在变态期间在中肠和丝腺中至少出现两个高峰。caspase-1 转录本在 caspase 中表达水平最高，而 caspase-3 和 caspase-6 似乎是与变态期间 IAP 最相关的 caspase，表明 IAP 和 caspase 可能参与了大蜡螟中与苍蝇和蚊子相同的核心凋亡途径。
