Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan.
Biol Pharm Bull. 2012;35(9):1409-12. doi: 10.1248/bpb.b212010.
In many diseases (e.g. autoimmune diseases, cancer), a humoral immune response is thought to be involved; therefore, identifying relevant antigens that trigger the humoral immune response is at the heart of current approaches for discovering biomarkers and designing therapeutic strategies. Although immunoblotting and protein array have been used for antigen profiling in several diseases, with the rapid progress in mass spectrometry (MS) and separation technologies, MS-based proteomic methods have become a promising tool for identifying disease-associated antigens. This review introduces MS-based antigen profiling methods (immunoproteomics and immune complexome analysis) and presents application examples of these techniques to the analysis of biological samples.
在许多疾病（例如自身免疫性疾病、癌症）中，人们认为体液免疫反应与之相关；因此，鉴定触发体液免疫反应的相关抗原是发现生物标志物和设计治疗策略的核心。尽管免疫印迹和蛋白质芯片已被用于几种疾病的抗原分析，但随着质谱（MS）和分离技术的快速发展，基于 MS 的蛋白质组学方法已成为鉴定疾病相关抗原的一种有前途的工具。本文介绍了基于 MS 的抗原分析方法（免疫蛋白质组学和免疫复合物组分析），并展示了这些技术在生物样本分析中的应用实例。
