Katiyar Madhu, Ali Amjad
School of Chemistry and Biochemistry, Thapar University, Patiala, India.
J Oleo Sci. 2012;61(9):469-75. doi: 10.5650/jos.61.469.
Present study demonstrated the preparation of MCM-41 as a support for the immobilization of Candida rugosa lipase by the physical adsorption technique. The lipase immobilized MCM-41 has been characterized by scanning electron microscopic and FTIR techniques. At pH 6, maximum lipase immobilization (250 mg/g) on MCM support has been observed and the immobilized lipase was employed as biocatalyst for the transesterification of the cotton seed oil with methanol. The pH of the reaction medium, reaction temperature and methanol/oil molar ratio have been optimized to achieve a maximum 98±3% fatty acid methyl esters yield (FAMEs)from cotton seed oil.
本研究展示了通过物理吸附技术制备MCM-41作为固定化皱落假丝酵母脂肪酶的载体。固定化脂肪酶的MCM-41已通过扫描电子显微镜和傅里叶变换红外光谱技术进行了表征。在pH 6时，观察到MCM载体上脂肪酶的最大固定化量（250 mg/g），并且固定化脂肪酶被用作棉籽油与甲醇进行酯交换反应的生物催化剂。已对反应介质的pH、反应温度和甲醇/油摩尔比进行了优化，以实现从棉籽油中获得最高98±3%的脂肪酸甲酯产率（FAMEs）。
