Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany.
Clin Hemorheol Microcirc. 2012;52(2-4):313-23. doi: 10.3233/CH-2012-1607.
Stent thrombosis and restenosis after drug-eluting stent (DES) implantation remains a relevant problem in the cardiovascular field. The polymer-based biomaterial (e.g. stent coating) requirements are comprehensive, since the polymeric material ideally should ensure an effective re-endothelialization by recruiting endothelial cells (EC) and endothelial progenitor cells (EPC). Simultaneously, the polymer should effectively prevent adherence of smooth muscle cells (SMC) and thereby inhibiting restenosis. The aim of this study was to gain a basic understanding on the interaction of SMC and human umbilical vein endothelial cells (HUVEC) with nonporous polymer films. A multifunctional copolyetheresterurethane (PDC) was chosen as candidate material: PDC consists of poly(p-dioxanone) (PPDO) and poly(ε-caprolactone)-segments (PCL). In our study it was compared to the degradable PPDO homopolymer and poly(vinylidene fluoride-co-hexafluoropropene) (PVDF), an established coating material of DES in clinical applications intended for longterm applications. The films were analyzed according to their thermomechanical and surface properties before being examined in contact with HUVEC and SMC concerning cell viability, proliferation and adhesion. Experimental results showed that adhesion could be improved for HUVEC on PDC compared to PPDO and PVDF. In contrast, SMC attachment is largely suppressed on PDC polymeric films indicating a cell-specific response of HUVEC towards PDC. In conclusion, PDC represents a promising candidate material for future cardiovascular applications like e.g. biodegradable (PDC) stent coatings.
药物洗脱支架（DES）植入后支架内血栓形成和再狭窄仍然是心血管领域的一个相关问题。基于聚合物的生物材料（例如支架涂层）的要求是全面的，因为理想情况下，聚合物材料应通过招募内皮细胞（EC）和内皮祖细胞（EPC）来确保有效的再内皮化。同时，聚合物应有效地防止平滑肌细胞（SMC）的黏附，从而抑制再狭窄。本研究的目的是深入了解SMC和人脐静脉内皮细胞（HUVEC）与无孔聚合物薄膜的相互作用。选择多功能共聚醚酯聚氨酯（PDC）作为候选材料：PDC 由聚（对二氧环己酮）（PPDO）和聚（ε-己内酯）段（PCL）组成。在我们的研究中，它与可降解的 PPDO 均聚物和聚（偏二氟乙烯-共-六氟丙烯）（PVDF）进行了比较，PVDF 是临床应用中用于长期应用的 DES 的一种既定涂层材料。在与 HUVEC 和 SMC 接触以研究细胞活力、增殖和黏附之前，根据其热机械和表面性能对薄膜进行了分析。实验结果表明，与 PPDO 和 PVDF 相比，PDC 可提高 HUVEC 的黏附能力。相比之下，PDC 聚合物薄膜上的 SMC 黏附被大大抑制，这表明 HUVEC 对 PDC 具有细胞特异性反应。总之，PDC 是一种有前途的候选材料，可用于未来的心血管应用，例如可生物降解的（PDC）支架涂层。
