Ibuki Yuko, Toyooka Tatsushi
Institute for Environmental Sciences, University of Shizuoka, Shizuoka-shi, Japan.
Methods Mol Biol. 2012;926:157-66. doi: 10.1007/978-1-62703-002-1_11.
The uptake of nanoparticles by cells is an important factor to assess nanotoxicity. In general, the nanoparticles taken up by the cells have been identified by transmission electron microscope, inductively coupled plasma mass spectrometry, etc.; however, the methods required an immense amount of time and effort. Flow cytometry (FCM) has been used and developed in the fields of biochemistry and clinical hematology, and has advantages to analyze thousands of cells in seconds. We recently clarified that the side-scatter(ed) light of FCM could be used as a guide to measure uptake potential of nanoparticles. Here, we describe the protocol for screening of the uptake potential of nanoparticles using FCM.
细胞对纳米颗粒的摄取是评估纳米毒性的一个重要因素。一般来说，细胞摄取的纳米颗粒已通过透射电子显微镜、电感耦合等离子体质谱等方法进行鉴定；然而，这些方法需要大量的时间和精力。流式细胞术（FCM）已在生物化学和临床血液学领域得到应用和发展，具有在几秒钟内分析数千个细胞的优势。我们最近阐明，FCM的侧向散射光可作为测量纳米颗粒摄取潜力的指导。在此，我们描述了使用FCM筛选纳米颗粒摄取潜力的方案。
