Tůma Petr, Stulík Karel
Institute of Biochemistry, Cell and Molecular Biology, Charles University, Prague, Czech Republic.
Methods Mol Biol. 2013;919:13-23. doi: 10.1007/978-1-62703-029-8_2.
Capillary electrophoresis (CE) with contactless conductivity detection (C(4)D) is readily applicable to determinations of amino acids in clinical samples. Most of these analyses employ long separation pathways. This chapter describes CE/C(4)D determinations of 28 biogenic amino acids in a short capillary with an effective length of 18 cm. All the test amino acids can be mutually separated in electrolytes of 0.5-10 mol/L acetic acid. The time of analysis does not exceed 6 min; the limits of detection vary from 0.1 to 1.7 μmol/L for all the analytes. The pretreatment of the biological material is very simple, consisting of the removal of proteins by an addition of acetonitrile and subsequent filtration. The procedure has been successfully applied to determinations of the whole amino acid spectra in blood plasma, urine, saliva, cerebrospinal, and amniotic fluid samples.
具有非接触式电导检测（C(4)D）的毛细管电泳（CE）很容易应用于临床样本中氨基酸的测定。这些分析大多采用长分离路径。本章描述了在有效长度为18 cm的短毛细管中对28种生物源性氨基酸进行CE/C(4)D测定。所有测试的氨基酸在0.5 - 10 mol/L乙酸电解质中均可相互分离。分析时间不超过6分钟；所有分析物的检测限在0.1至1.7 μmol/L之间。生物材料的预处理非常简单，包括加入乙腈去除蛋白质并随后过滤。该方法已成功应用于血浆、尿液、唾液、脑脊液和羊水样本中全氨基酸谱的测定。
