基于微芯片电泳和化学发光检测的甲状腺素均相免疫分析方法。
A homogeneous immunoassay of thyroxine based on microchip electrophoresis and chemiluminescence detection.
作者信息
Zhao Shulin, Liu Yi-Ming
机构信息
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, China.
出版信息
Methods Mol Biol. 2013;919:79-85. doi: 10.1007/978-1-62703-029-8_8.
Abstract
A homogeneous chemiluminescent immunoassay of thyroxine (T4) present in serum samples is described. The proposed method deployed the competitive immunoreaction of T4 and horseradish peroxidase (HRP)-labeled T4 (HRP-T4) with anti-T4 mouse monoclonal antibody (Ab). HRP-T4 and the HRP-T4-Ab complex were separated and quantified by using microchip electrophoresis (MCE) with chemiluminescence (CL) detection. The MCE separation was accomplished within 60 s. Highly sensitive CL detection was achieved by means of HPR-catalyzed luminol-H(2)O(2) reaction. The linear range for T4 was 5-250 nM with a detection limit of 2.2 nM (S/N = 3).
摘要
本文描述了一种用于检测血清样本中甲状腺素（T4）的均相化学发光免疫分析方法。该方法利用T4与辣根过氧化物酶（HRP）标记的T4（HRP-T4）与抗T4小鼠单克隆抗体（Ab）之间的竞争性免疫反应。通过使用带有化学发光（CL）检测的微芯片电泳（MCE）对HRP-T4和HRP-T4-Ab复合物进行分离和定量。MCE分离在60秒内完成。通过HPR催化的鲁米诺-H2O2反应实现了高灵敏度的CL检测。T4的线性范围为5-250 nM，检测限为2.2 nM（S/N = 3）。
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