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微流控芯片上的电泳分离

Electrophoretic separations on microfluidic chips.

作者信息

Wu Dapeng, Qin Jianhua, Lin Bingcheng

机构信息

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

出版信息

J Chromatogr A. 2008 Mar 14;1184(1-2):542-59. doi: 10.1016/j.chroma.2007.11.119. Epub 2007 Dec 23.

DOI:10.1016/j.chroma.2007.11.119
PMID:18207148
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7094303/
Abstract

This review presents a brief outline and novel developments of electrophoretic separation in microfluidic chips. Distinct characteristics of microchip electrophoresis (MCE) are discussed first, in which sample injection plug, joule heat, channel turn, surface adsorption and modification are introduced, and some successful strategies and recognized conclusions are also included. Important achievements of microfluidic electrophoresis separation in small molecules, DNA and protein are then summarized. This review is aimed at researchers, who are interested in MCE and want to adopt MCE as a functional unit in their integrated microsystems.

摘要

本综述介绍了微流控芯片中电泳分离的简要概述和新进展。首先讨论了微芯片电泳（MCE）的独特特性，其中介绍了样品进样塞、焦耳热、通道转弯、表面吸附和修饰，还包括一些成功的策略和公认的结论。然后总结了微流控电泳分离在小分子、DNA和蛋白质方面的重要成果。本综述针对那些对MCE感兴趣并希望在其集成微系统中采用MCE作为功能单元的研究人员。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/2ebe41305361/gr12.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/0886fc19c074/gr6.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/9dd1341d5fb1/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/2ebe41305361/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/8b442b921b15/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/60736eeb0b90/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/eab7fc074f61/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/dc0009306e44/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/25609d46e28d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/0886fc19c074/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/70b89f2fae28/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/31d01f309a5d/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/9cdf86ff9d5f/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/c2d6f80eb2e8/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/9dd1341d5fb1/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0f8/7094303/2ebe41305361/gr12.jpg

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1
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2
Subattomole-Sensitivity Microchip Nanoelectrospray Source with Time-of-Flight Mass Spectrometry Detection.具有飞行时间质谱检测功能的亚阿托摩尔灵敏度微芯片纳电喷雾源
Anal Chem. 1999 Sep 1;71(17):3627-31. doi: 10.1021/ac990373m.
3
Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane).聚二甲基硅氧烷微流控系统的快速成型
用于提高小分子回收率的聚二甲基硅氧烷微流控装置表面处理的评估及其在监测胰岛分泌代谢物中的应用
ACS Meas Sci Au. 2023 Aug 24;3(5):380-389. doi: 10.1021/acsmeasuresciau.3c00025. eCollection 2023 Oct 18.
4
Technological advances for analyzing the content of organ-on-a-chip by mass spectrometry.用于通过质谱分析芯片上器官内容物的技术进展。
Front Bioeng Biotechnol. 2023 May 22;11:1197760. doi: 10.3389/fbioe.2023.1197760. eCollection 2023.
5
Microscale Diffusiophoresis of Proteins.蛋白质的微观扩散电泳。
J Phys Chem B. 2022 Nov 10;126(44):8913-8920. doi: 10.1021/acs.jpcb.2c04029. Epub 2022 Oct 28.
6
Microfluidics for High Pressure: Integration on GaAs Acoustic Biosensors with a Leakage-Free PDMS Based on Bonding Technology.用于高压的微流体：基于键合技术在GaAs声学生物传感器上集成无泄漏聚二甲基硅氧烷
Micromachines (Basel). 2022 May 11;13(5):755. doi: 10.3390/mi13050755.
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Addressing the global challenges of COVID-19 and other pulmonary diseases with microfluidic technology.利用微流控技术应对新冠疫情及其他肺部疾病的全球挑战。
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4
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5
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6
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