Ryzhikov Mikhail, Korolev Sergey
Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, St. Louis, MO, USA.
Methods Mol Biol. 2012;922:123-31. doi: 10.1007/978-1-62703-032-8_7.
Interaction of recombination protein RecO with single-stranded (ss) DNA-binding protein (SSB) is essential for DNA damage repair and restart of stalled replication (Cox, Crit Rev Biochem Mol Biol 42(1):41-63, 2007). To understand mechanism of this interaction and its role in DNA repair, we deciphered a high-resolution structure of RecO complex with C-terminal tail of SSB (SSB-Ct). The structure revealed a key role of hydrophobic interactions between two proteins and suggests the mechanism of RecO recruitment to DNA during homologous recombination and strand annealing.
