Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences, Umeå, Sweden.
Fish Physiol Biochem. 2013 Jun;39(3):471-8. doi: 10.1007/s10695-012-9712-3. Epub 2012 Sep 14.
Male goldfish (Carassius auratus) exposed to female hormonal pheromones express increased milt volumes and their sperm fertilize more eggs than sperm from unprimed males. Ovulated salmonid females also release odours that increase volumes of strippable milt in males. It is, however, not known if the priming pheromones affect the ability of sperm to fertilize eggs in salmonids. In this study, we compare the proportion of larvae produced from in vitro fertilization tests between primed brown trout (Salmo trutta) males exposed to a mix of female urine and ovarian fluids, and control males exposed only to 0.9 % sodium chloride. We also investigate priming effects on milt yield and sperm motility. Fertilization tests with sperm from single males, as well as sperm from two males (i.e., sperm competition), were performed. Primed males generated more larvae in both the single male and competition fertilization tests. No differences between treatments in milt yield and sperm motility could be established.
雄性金鱼（Carassius auratus）暴露于雌性激素信息素后，精液量增加，其精子比未经诱导的雄性金鱼的精子能使更多的卵子受精。已排卵的鲑鱼雌鱼也会释放气味，增加雄性鱼可挤出精液的量。然而，目前尚不清楚诱导信息素是否会影响鲑鱼精子使卵子受精的能力。在这项研究中，我们比较了暴露于雌性尿液和卵巢液混合物的雄性棕色鳟鱼（Salmo trutta）与仅暴露于 0.9%氯化钠的对照组雄性鱼的体外受精试验中产生的幼虫比例。我们还研究了诱导对精液量和精子活力的影响。对单只雄性鱼的精子和两只雄性鱼的精子（即精子竞争）进行了受精试验。在单只雄性和竞争受精试验中，诱导后的雄性鱼产生的幼虫更多。在精液量和精子活力方面，处理组之间没有差异。
