Dpto. Biología de Organismos, Universidad Simón Bolívar, Baruta, Apartado 89000, Caracas 1080, Venezuela.
Bull Environ Contam Toxicol. 2012 Nov;89(5):1068-72. doi: 10.1007/s00128-012-0820-7. Epub 2012 Sep 14.
Our purpose was to detect, isolate and characterize tensioactive agents with or without emulsifying activity from marine bacterial strains present in seawater and sediment samples from the Venezuelan Atlantic Front. Biosurfactants found in cell-free supernatants from all cultures presented high surface activity as they were able to reduce the water surface tension from 72 dynes cm(-1) to values between 41.7 and 33.9 dynes cm(-1). However, high indirect CMC values were registered for the most of these compounds (51.4%-56.1% v/v). Culture supernatants from S3 and S29 strains showed highest emulsifying activity in the dispersion assay with diesel oil (absorbance 1.06 and 1.18, respectively), while supernatant from the S10 strain induced the most stable oil-in-water emulsions with 16° and 25°API crude oils. Only culture supernatant from S3 strain was able to produce stable oil-in water emulsions with diesel oil and both type of crude oils.
我们的目的是从委内瑞拉大西洋前缘的海水和沉积物样本中分离和鉴定具有或不具有乳化活性的海洋细菌菌株中的表面活性剂。所有培养物的无细胞上清液中发现的生物表面活性剂具有很高的表面活性，因为它们能够将水的表面张力从 72 达因/厘米(-1)降低到 41.7 到 33.9 达因/厘米(-1)之间。然而，这些化合物中的大多数都具有较高的间接 CMC 值（51.4%-56.1% v/v）。S3 和 S29 菌株的培养上清液在柴油分散试验中表现出最高的乳化活性（分别为吸光度 1.06 和 1.18），而 S10 菌株的上清液则能够形成最稳定的 16°和 25°API 原油的油包水乳状液。只有 S3 菌株的培养上清液能够与柴油和两种类型的原油形成稳定的油包水乳状液。
