He Zengyou, Qi Robert Z, Yu Weichuan
School of Software, Dalian University of Technology, Dalian, China.
Top Curr Chem. 2013;331:193-209. doi: 10.1007/128_2012_365.
In this chapter we first describe the applications of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) in biomarker discovery. After a summary of the general analysis pipeline of MALDI MS data, each step of the pipeline will be elaborated in detail. In particular we try to provide a categorization of existing solutions with the hope that the reader can obtain a global picture on this topic. In addition we show how to apply such an analysis pipeline in protein and glycan profiling for biomarker discovery and for a deeper understanding of diseases. Finally we discuss the limitations of current analysis methods and the perspectives of future research.
在本章中，我们首先描述基质辅助激光解吸/电离（MALDI）质谱（MS）在生物标志物发现中的应用。在总结MALDI MS数据的一般分析流程后，将详细阐述该流程的每一步。特别是，我们试图对现有解决方案进行分类，希望读者能对该主题有一个全面的了解。此外，我们展示了如何将这样的分析流程应用于蛋白质和聚糖分析，以发现生物标志物并更深入地了解疾病。最后，我们讨论了当前分析方法的局限性以及未来研究的前景。
