Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110 016, India.
Appl Biochem Biotechnol. 2012 Nov;168(6):1655-63. doi: 10.1007/s12010-012-9886-2. Epub 2012 Sep 14.
The effect of culture temperature on urokinase production by HT-1080 cell line was studied in batch culture and hollow fiber reactor. Small-scale t-flask experiments revealed that urokinase production could be enhanced and media utilization could be reduced by lowering the culture temperature in production phase. Urokinase production was scaled up using a hollow fiber perfusion reactor system. Temperature of culture was maintained at the physiological 37 °C during growth phase that extended up to 12 days in hollow fiber bioreactor. Subsequently, in the production phase, culture temperature was lowered to 34 °C. Decrease in culture temperature resulted in a significant increase in urokinase production. Proteolytic degradation and inhibition was also minimized. The medium utilization rate was decreased at lower temperature, and hence, a higher economy of production could be obtained.
研究了在分批培养和中空纤维反应器中，培养温度对 HT-1080 细胞系尿激酶生产的影响。小瓶实验表明，降低生产阶段的培养温度可以提高尿激酶的产量并减少培养基的利用。使用中空纤维灌注反应器系统对尿激酶进行了放大。在中空纤维生物反应器中，培养温度在生长阶段保持在生理温度 37°C，持续 12 天。随后，在生产阶段，将培养温度降低到 34°C。降低培养温度可显著提高尿激酶的产量，并最大限度地减少蛋白水解降解和抑制。在较低温度下，培养基的利用率降低，因此可以获得更高的生产经济性。
