Khaparde Shilpa S, Roychoudhury Pradip K
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, New Delhi, India,
J Ind Microbiol Biotechnol. 2014 Jun;41(6):1035-8. doi: 10.1007/s10295-014-1434-3. Epub 2014 Mar 27.
Medium optimization is an important strategy that can lead to several fold increase in the production of proteins in cell culture. However, the usual methods of medium optimization are complex and time consuming. Urokinase is a widely employed thrombolytic drug for the treatment of stroke. We describe here medium optimization for maximizing urokinase production by HT-1080 cells using supplementation of specific amino acids. The new specifically designed method resulted in 240 % increase in urokinase productivity.
培养基优化是一种重要策略，可使细胞培养中蛋白质产量提高数倍。然而，常规的培养基优化方法复杂且耗时。尿激酶是一种广泛用于治疗中风的溶栓药物。我们在此描述通过添加特定氨基酸来优化培养基，以实现HT - 1080细胞尿激酶产量最大化。这种新的专门设计方法使尿激酶生产率提高了240%。
