Goffney W H, Wong J H, Kern D H, Chase D, Krag D N, Storm F K
John Wayne Cancer Clinic, Armand Hammer Laboratories, Jonsson Comprehensive Cancer Center, UCLA School of Medicine.
Cancer Res. 1990 Feb 1;50(3):459-63.
Because both Rhodamine 123 (R123) and hyperthermia have been shown to be cytotoxic, we examined their effect, independently and in combination, on five different human malignant cell lines in vitro and on cultured melanoma cells grown intradermally in nude mice. The cell lines examined include two human melanomas, UCLA-SO-M14 and UCLA-SO-M21, the colon cancer cell line HT29, the human lung cancer cell line P3, and the human breast cancer cell line B231. R123 and hyperthermia, when used in combination, were found to be cytotoxic for these five different human malignant cell lines in vitro. The two agents together appear to enhance the cytotoxic effect of each alone, as documented by synergistic ratios ranging from 2.31 to 45 for the different cell lines. In the "nude" mouse model, animals were treated with a combination of R123 and hyperthermia (43 degrees C for 90 min). A statistically significant (P = 0.04) decrease in tumor growth rate was observed when compared with the rate of tumor growth in untreated animals. The results suggest a potential role for R123 in combination with hyperthermia in the treatment of malignant cells.
由于已证明罗丹明123（R123）和热疗均具有细胞毒性，我们分别及联合检测了它们对五种不同人类恶性细胞系的体外作用，以及对裸鼠皮内生长的培养黑色素瘤细胞的作用。所检测的细胞系包括两种人类黑色素瘤细胞系UCLA-SO-M14和UCLA-SO-M21、结肠癌细胞系HT29、人类肺癌细胞系P3以及人类乳腺癌细胞系B231。结果发现，R123和热疗联合使用时，对这五种不同人类恶性细胞系具有体外细胞毒性。两种药物联合使用似乎增强了各自单独使用时的细胞毒性作用，不同细胞系的协同比在2.31至45之间，证明了这一点。在“裸”鼠模型中，动物接受了R123和热疗（43℃，90分钟）的联合治疗。与未治疗动物的肿瘤生长速率相比，观察到肿瘤生长速率有统计学意义的下降（P = 0.04）。结果表明，R123与热疗联合在治疗恶性细胞方面具有潜在作用。
