Nucleic Acid Chemistry Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Chem Biodivers. 2012 Sep;9(9):2000-7. doi: 10.1002/cbdv.201100425.
ICON Probes, short DNA strands containing an adenine linked to a bipyridine ligand, formed an interstrand cross-link with 5-methylcytosine located opposite the modified adenine in the presence of an osmium oxidant. The location of a bipyridine-tethered adenine in the probes varied the selectivity of the reactive base. An ICON probe where the modified adenine was located at the probe center showed a 5-methylcytosine-selective osmium complexation, whereas an ICON probe with the modified adenine at the strand end exhibited high reactivity towards thymine as well as 5-methylcytosine. The modulation of reactive bases by the incorporation of a bipyridine-tethered adenine site made facilitates design of ICON probes for the fluorometric detection of 5-methylcytosine.
ICON 探针是含有腺嘌呤与联吡啶配体相连的短 DNA 链，在锇氧化剂存在下，与位于修饰腺嘌呤对面的 5-甲基胞嘧啶形成链间交叉连接。探针中连接联吡啶的腺嘌呤的位置改变了反应碱基的选择性。位于探针中心的修饰腺嘌呤的 ICON 探针显示出 5- 甲基胞嘧啶选择性的锇络合物形成，而在链端具有修饰腺嘌呤的 ICON 探针对胸腺嘧啶以及 5- 甲基胞嘧啶表现出高反应性。通过引入联吡啶连接的腺嘌呤位点来调节反应碱基，使得设计用于荧光检测 5- 甲基胞嘧啶的 ICON 探针变得更加容易。
