Tanaka Kazuo, Tainaka Kazuki, Okamoto Akimitsu
Frontier Research System, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Nucleic Acids Symp Ser (Oxf). 2006(50):139-40. doi: 10.1093/nass/nrl069.
We report the control of fluorescence emission from the fluorophore fixed on DNA using the methylcytosine-selective addition of an osmium(VI)-bipyridine complex. We synthesized the DNA modified by a microenvironment-sensitive fluorophore, 2-dimethylamino-6-acylnaphthalene. The fluorescence from the fluorophore tethered to a probe DNA was effectively quenched by the addition of the osmium(VIII)-bipyridine to the methylcytosine which is located at the immediate neighborhood of the fluorophore. The discrimination of cytosine methylation status at the mutation hot spot in p53 gene was also executed using a well-designed fluorescent DNA probe.
我们报道了通过甲基胞嘧啶选择性添加锇（VI）-联吡啶配合物来控制固定在DNA上的荧光团的荧光发射。我们合成了由微环境敏感荧光团2-二甲基氨基-6-酰基萘修饰的DNA。通过向位于荧光团紧邻位置的甲基胞嘧啶添加锇（VIII）-联吡啶，有效地猝灭了连接到探针DNA上的荧光团的荧光。还使用精心设计的荧光DNA探针实现了对p53基因中突变热点处胞嘧啶甲基化状态的区分。
