锇配合物对单碱基对错配中甲基胞嘧啶的选择性氧化

Selective oxidation of methylcytosine in single base pair mismatches by osmium complex.

作者信息

Nomura Akiko, Tainaka Kazuki, Okamoto Akimitsu

机构信息

Frontier Research System, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2007(51):299-300. doi: 10.1093/nass/nrm150.

DOI:10.1093/nass/nrm150

PMID:18029705

Abstract

We have reported a novel method for methylcytosine (M)-positive epigenotyping, in which an osmium ion site-specifically binds to M in a single strand or a bulge structure of DNA on oxidation of an M C5-C6 double bond. Herein, we have examined the osmium oxidation reactivity of M that formed a mismatched base pair with various bases, and observed its unique reactivity.

摘要

我们报道了一种用于甲基胞嘧啶（M）阳性表观基因分型的新方法，其中锇离子在M的C5-C6双键氧化时位点特异性地与DNA单链或凸起结构中的M结合。在此，我们研究了与各种碱基形成错配碱基对的M的锇氧化反应性，并观察到其独特的反应性。