Sugaya-Fukasawa Maki, Watanabe Toru, Tamura Michiko, Egashira Satsuki, Hisatomi Hisashi
Laboratory of Cellular and Molecular Biochemistry, Department of Materials and Life Science, Seikei Universty, Musashino-shi, Tokyo 180-8633, Japan.
Exp Ther Med. 2011 Jan;2(1):85-87. doi: 10.3892/etm.2010.162. Epub 2010 Nov 5.
Nerve growth factor (NGF) normally induces the differentiation of PC12 cells into a neuron-like phenotype. In this study, we found that exposure of PC12 cells to a temperature of 42°C for 24 h significantly decreased NGF-induced neurite elongation. Glial fibrillary acidic protein (GFAP) levels were decreased when PC12 cells were exposed to the heat stress, while PC12 cells overexpressing the gene encoding GFAP showed resistance. Mock-transfected PC12 cells exposed to NGF could be cultured for 72 h at 37°C, whereas GFAP-transfected PC12 cells exposed to NGF could be cultured for over 100 h. Our results suggest that GFAP is necessary for the long-term maintenance of cells with a neuron-like phenotype.
神经生长因子（NGF）通常诱导PC12细胞分化为神经元样表型。在本研究中，我们发现将PC12细胞暴露于42°C温度下24小时会显著降低NGF诱导的神经突伸长。当PC12细胞受到热应激时，胶质纤维酸性蛋白（GFAP）水平降低，而过度表达编码GFAP基因的PC12细胞则表现出抗性。暴露于NGF的mock转染PC12细胞在37°C下可培养72小时，而暴露于NGF的GFAP转染PC12细胞可培养超过100小时。我们的结果表明，GFAP对于具有神经元样表型的细胞的长期维持是必需的。
