ENOD12基因产物参与豌豆与根瘤菌相互作用期间的感染过程。

The ENOD12 gene product is involved in the infection process during the pea-Rhizobium interaction.

作者信息

Scheres B, Van De Wiel C, Zalensky A, Horvath B, Spaink H, Van Eck H, Zwartkruis F, Wolters A M, Gloudemans T, Van Kammen A

机构信息

Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands.

出版信息

Cell. 1990 Jan 26;60(2):281-94. doi: 10.1016/0092-8674(90)90743-x.

DOI:10.1016/0092-8674(90)90743-x

PMID:2297789

Abstract

The pea cDNA clone pPsENOD12 represents a gene involved in the infection process during Pisum sativum L.-Rhizobium leguminosarum bv. viciae symbiosis. The ENOD12 protein is composed of pentapeptides containing two hydroxyprolines. The expression of the ENOD12 gene is induced in cells through which the infection thread is migrating, but also in cells that do not yet contain an infection thread. Soluble compounds from Rhizobium are involved in eliciting ENOD12 gene expression. Rhizobium common and host-specific nodulation genes are essential for the production of these compounds. Two ENOD12 genes are expressed in nodules and in stem tissue of uninoculated plants. The gene represented by the cloned ENOD12 mRNA is also expressed in flowers, but a different transcription start may be used.

摘要

豌豆cDNA克隆pPsENOD12代表了一个参与豌豆（Pisum sativum L.）-豌豆根瘤菌蚕豆生物型共生过程中感染进程的基因。ENOD12蛋白由含有两个羟脯氨酸的五肽组成。ENOD12基因的表达在感染丝穿过的细胞中被诱导，同时也在尚未含有感染丝的细胞中被诱导。来自根瘤菌的可溶性化合物参与引发ENOD12基因的表达。根瘤菌通用和宿主特异性结瘤基因对于这些化合物的产生至关重要。两个ENOD12基因在未接种植物的根瘤和茎组织中表达。由克隆的ENOD12 mRNA所代表的基因也在花中表达，但可能使用了不同的转录起始位点。