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ENOD12基因产物参与豌豆与根瘤菌相互作用期间的感染过程。

The ENOD12 gene product is involved in the infection process during the pea-Rhizobium interaction.

作者信息

Scheres B, Van De Wiel C, Zalensky A, Horvath B, Spaink H, Van Eck H, Zwartkruis F, Wolters A M, Gloudemans T, Van Kammen A

机构信息

Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands.

出版信息

Cell. 1990 Jan 26;60(2):281-94. doi: 10.1016/0092-8674(90)90743-x.

DOI:10.1016/0092-8674(90)90743-x
PMID:2297789
Abstract

The pea cDNA clone pPsENOD12 represents a gene involved in the infection process during Pisum sativum L.-Rhizobium leguminosarum bv. viciae symbiosis. The ENOD12 protein is composed of pentapeptides containing two hydroxyprolines. The expression of the ENOD12 gene is induced in cells through which the infection thread is migrating, but also in cells that do not yet contain an infection thread. Soluble compounds from Rhizobium are involved in eliciting ENOD12 gene expression. Rhizobium common and host-specific nodulation genes are essential for the production of these compounds. Two ENOD12 genes are expressed in nodules and in stem tissue of uninoculated plants. The gene represented by the cloned ENOD12 mRNA is also expressed in flowers, but a different transcription start may be used.

摘要

豌豆cDNA克隆pPsENOD12代表了一个参与豌豆(Pisum sativum L.)-豌豆根瘤菌蚕豆生物型共生过程中感染进程的基因。ENOD12蛋白由含有两个羟脯氨酸的五肽组成。ENOD12基因的表达在感染丝穿过的细胞中被诱导,同时也在尚未含有感染丝的细胞中被诱导。来自根瘤菌的可溶性化合物参与引发ENOD12基因的表达。根瘤菌通用和宿主特异性结瘤基因对于这些化合物的产生至关重要。两个ENOD12基因在未接种植物的根瘤和茎组织中表达。由克隆的ENOD12 mRNA所代表的基因也在花中表达,但可能使用了不同的转录起始位点。

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The ENOD12 gene product is involved in the infection process during the pea-Rhizobium interaction.ENOD12基因产物参与豌豆与根瘤菌相互作用期间的感染过程。
Cell. 1990 Jan 26;60(2):281-94. doi: 10.1016/0092-8674(90)90743-x.
2
Characterization of the pea ENOD12B gene and expression analyses of the two ENOD12 genes in nodule, stem and flower tissue.豌豆ENOD12B基因的特征分析以及两个ENOD12基因在根瘤、茎和花组织中的表达分析。
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[Pea (Pisum sativum) genes, participating in symbiosis with nitrogen-fixing bacteria. III. Study of the structure of the ENOD12 early nodulin gene for various types of peas using the polymerase chain reaction (PCR)].[豌豆(Pisum sativum)与固氮细菌共生相关基因。III. 利用聚合酶链反应(PCR)对不同类型豌豆中ENOD12早期结瘤素基因结构的研究]
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Sequential induction of nodulin gene expression in the developing pea nodule.豌豆根瘤发育过程中结瘤素基因表达的顺序诱导
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A novel type of DNA-binding protein interacts with a conserved sequence in an early nodulin ENOD12 promoter.一种新型的DNA结合蛋白与早期结瘤素ENOD12启动子中的保守序列相互作用。
Plant Mol Biol. 1996 Dec;32(5):809-21. doi: 10.1007/BF00020479.
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A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and nod factor induced expression.
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Identification of two alfalfa early nodulin genes with homology to members of the pea Enod12 gene family.鉴定出两个与豌豆Enod12基因家族成员具有同源性的苜蓿早期结瘤素基因。
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Plant J. 1993 Oct;4(4):727-33. doi: 10.1046/j.1365-313x.1993.04040727.x.

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