Zhang Hongcai, He Lin, Li Huiyun, Tao Nengfu, Chang Tianda, Wang Dongmei, Lu Yichu, Li Zhenying, Mai Chunhai, Zhao Xiaorui, Niu Bingjie, Ma Junkui, Wang Lixiang
Shanxi Houji Laboratory, College of Agriculture, Shanxi Agricultural University, Taigu 030801, China.
Int J Mol Sci. 2025 Jan 21;26(3):879. doi: 10.3390/ijms26030879.
Symbiotic nitrogen fixation, recognized as the most efficient nitrogen assimilation system in ecosystems, is essential for soybean growth, as nodulation provides critical nitrogen to host cells. Soybeans thrive in warm and moist environments. However, they are highly susceptible to low temperatures, which impede the formation and development of root nodules. The genetic basis and molecular mechanism underlying the inhibition of nodulation induced by low temperatures remain unclear. In this study, we conducted a comparative transcriptomic analysis of soybean roots inoculated with rhizobium at 1 DPI (Day Post Inoculation) under normal or cold treatments. We identified 39 up-regulated and 35 down-regulated genes associated with nodulation and nitrogen fixation. Notably, cold-responsive genes including three () family genes were identified among differentially expressed genes (DEGs). Further expression pattern analysis of demonstrated it being significantly responsive to rhizobium inoculation and its highest expression in nodules. Further investigation revealed that overexpression of led to an increase in the nodule number, while RNA interference (RNAi)-mediated gene editing of suppressed nodule formation. Additionally, overexpression may regulate soybean nodulation by modulating the expression of (NODULE INCEPTION), (nodulation signaling pathway 1), and (histone- or haem-associated protein domain) in the nod factor signaling pathway. This study offers new insights into the genetic basis of nodulation regulation under cold stress in legumes and indicates that may serve as a key regulator of nodule formation under cold stress.
共生固氮被认为是生态系统中最有效的氮同化系统，对大豆生长至关重要，因为结瘤为宿主细胞提供关键的氮。大豆在温暖湿润的环境中茁壮成长。然而，它们对低温高度敏感，低温会阻碍根瘤的形成和发育。低温诱导结瘤抑制的遗传基础和分子机制尚不清楚。在本研究中，我们对在正常或冷处理下接种根瘤菌1天后的大豆根进行了比较转录组分析。我们鉴定出39个与结瘤和固氮相关的上调基因和35个下调基因。值得注意的是，在差异表达基因（DEGs）中鉴定出了包括三个（）家族基因在内的冷响应基因。对的进一步表达模式分析表明，它对根瘤菌接种有显著响应，并且在根瘤中表达最高。进一步研究发现，的过表达导致根瘤数量增加，而RNA干扰（RNAi）介导的基因编辑抑制了根瘤形成。此外，的过表达可能通过调节根瘤因子信号通路中的（结瘤起始）、（结瘤信号通路1）和（组蛋白或血红素相关蛋白结构域）的表达来调节大豆结瘤。本研究为豆科植物冷胁迫下结瘤调控的遗传基础提供了新的见解，并表明可能是冷胁迫下根瘤形成的关键调节因子。
