补肺健脾方对慢性阻塞性肺疾病大鼠膈神经放电及膈肌功能的影响

[Effects of Bufei Jianpi Recipe on the diaphragmatic neural discharge and the diaphragmatic muscle function in rats with chronic obstructive pulmonary disease].

作者信息

Li Su-Yun, Li Ya, Li Jian-Sheng

机构信息

Department of Respiratory Diseases, First Affiliated Hospital of Henan College of Traditional Chinese Medicine, Zhengzhou.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 Jun;32(6):812-6.

PMID:22978110

Abstract

OBJECTIVE

To observe the effects of Bufei Jianpi Recipe (BJR) on the diaphragmatic neural discharge and the diaphragmatic muscle function in rats with chronic obstructive pulmonary disease (COPD).

METHODS

Rats were randomly divided into the normal control group, the model group, the high dose BJR group (9.68 g/kg x d(-1)), the medium dose BJR group (4.84 g/kg x d(-1)), the low dose BJR group (2.42 g/kg x d(-1)), and the aminophylline group (2.3 mg/kg x d(-1)). The stable phase COPD rat model was prepared using repeated smoke inhalations and bacterial infections. The high, medium, and low dose BJR and aminophylline was respectively administered to rats from the ninth week to the twentieth week. The sampling was taken. The lung function, diaphragmatic neural discharge time (Td), and diaphragmatic neural discharge interval (Tdi), diaphragmatic neural discharge range (Rd), diaphragmatic neural discharge area (Ad), expiratory time (Tex), inspiratory time (Tin), respiratory rate (RR), respiratory excursion (RE), respiratory area (RA), and diaphragmatic muscular tension and endurance were detected.

RESULTS

Compared with the normal control group, the tidal volume (TV), peak expiratory flow (PEF), and 50% tidal volume expiratory flow (EF50) significantly decreased in the model group (P < 0.01). Td, Tdi, Tex, and Tin were significantly prolonged (P < 0. 05, P < 0.01). Ad, Rd, RR, RE, RA, diaphragmatic muscular tension and endurance significantly decreased (P < 0.05, P < 0.01). The ratio of type I and IIA diaphragmatic fibers significantly increased and type IIB significantly decreased (P < 0.01). The activity of ATP decreased and the activity of SDH increased (P < 0.01). The aforesaid indices were improved to different degrees in BJR groups, especially in the high dose BJR group and the medium dose BJR group (P < 0.05, P < 0.01).

CONCLUSIONS

BJR could significantly improve the diaphragmatic neural discharge and the diaphragmatic muscle function. Its efficacy was better than that of aminophylline.

摘要

目的

观察补肺健脾方对慢性阻塞性肺疾病（COPD）大鼠膈神经放电及膈肌功能的影响。

方法

将大鼠随机分为正常对照组、模型组、补肺健脾方高剂量组（9.68 g/kg×d⁻¹）、补肺健脾方中剂量组（4.84 g/kg×d⁻¹）、补肺健脾方低剂量组（2.42 g/kg×d⁻¹）及氨茶碱组（2.3 mg/kg×d⁻¹）。采用反复烟熏加细菌感染的方法制备稳定期COPD大鼠模型。从第9周开始至第20周分别给予大鼠高、中、低剂量补肺健脾方及氨茶碱。进行取材，检测肺功能、膈神经放电时间（Td）、膈神经放电间隔（Tdi）、膈神经放电范围（Rd）、膈神经放电面积（Ad）、呼气时间（Tex）、吸气时间（Tin）、呼吸频率（RR）、呼吸动度（RE）、呼吸面积（RA）以及膈肌肌张力和耐力。

结果

与正常对照组比较，模型组潮气量（TV）、呼气峰流速（PEF）及50%潮气量呼气流量（EF50）显著降低（P<0.01）。Td、Tdi、Tex及Tin显著延长（P<0.05，P<0.01）。Ad、Rd、RR、RE、RA、膈肌肌张力和耐力显著降低（P<0.05，P<0.01）。膈肌Ⅰ型和ⅡA型纤维比例显著升高，ⅡB型显著降低（P<0.01）。ATP活性降低，SDH活性升高（P<0.01）。补肺健脾方各剂量组上述指标均有不同程度改善，尤以高剂量组和中剂量组明显（P<0.05，P<0.01）。