补肺益肾颗粒联合穴位贴敷疗法对慢性阻塞性肺疾病大鼠肺表面活性物质蛋白的影响

Effects of Bufei Yishen Granules Combined with Acupoint Sticking Therapy on Pulmonary Surfactant Proteins in Chronic Obstructive Pulmonary Disease Rats.

作者信息

Tian Yange, Li Jiansheng, Li Ya, Dong Yuqiong, Yao Fengjia, Mao Jing, Li Linlin, Wang Lili, Luo Shan, Wang Minghang

机构信息

Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Zhengzhou, Henan 450046, China; Institute for Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China; Institute for Respiratory Diseases, The First Affiliated Hospital, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450008, China.

出版信息

Biomed Res Int. 2016;2016:8786235. doi: 10.1155/2016/8786235. Epub 2016 Sep 6.

Our previous studies have demonstrated the beneficial effects of Bufei Yishen granules combined with acupoint sticking therapy (the integrated therapy) in chronic obstructive pulmonary disease (COPD), but the underlying mechanism remains unclear. Dysfunction of pulmonary surfactant proteins (SPs, including SP-A, SP-B, SP-C, and SP-D) may be included in pathophysiology of COPD. This study aimed to explore the mechanism of the integrated therapy on SPs. COPD rat models were established. The treatment groups received Bufei Yishen granules or acupoint sticking or their combination. Using aminophylline as a positive control drug. The levels of SPs in serum, BALF, and lung were measured. The results showed that the integrated therapy markedly reduced the levels of SPs in serum and increased these indicators in the lung. The integrated therapy was better than aminophylline in reducing the levels of SPs and was better than Bufei Yishen granules in reducing SP-A, SP-C, and SP-D in serum. The integrated therapy was better than aminophylline and Bufei Yishen granules in increasing SP-A, SP-B, and SP-D mRNA in the lung. SP-A and SP-D in BALF were positively correlated with PEF and EF50. The levels of SPs are associated with airway limitation. The beneficial effects of the integrated therapy may be involved in regulating pulmonary surfactant proteins.

我们之前的研究已经证明了补肺益肾颗粒联合穴位贴敷疗法（综合疗法）对慢性阻塞性肺疾病（COPD）的有益作用，但其潜在机制仍不清楚。肺表面活性物质蛋白（SPs，包括SP-A、SP-B、SP-C和SP-D）功能障碍可能参与了COPD的病理生理过程。本研究旨在探讨综合疗法对SPs的作用机制。建立COPD大鼠模型。治疗组分别给予补肺益肾颗粒、穴位贴敷或二者联合治疗。使用氨茶碱作为阳性对照药物。检测血清、支气管肺泡灌洗液（BALF）和肺组织中SPs的水平。结果显示，综合疗法显著降低了血清中SPs的水平，并提高了肺组织中这些指标的水平。在降低SPs水平方面，综合疗法优于氨茶碱，在降低血清中SP-A、SP-C和SP-D水平方面优于补肺益肾颗粒。在提高肺组织中SP-A、SP-B和SP-D mRNA水平方面，综合疗法优于氨茶碱和补肺益肾颗粒。BALF中的SP-A和SP-D与呼气峰流速（PEF）和50%用力呼气流量（EF50）呈正相关。SPs水平与气道受限有关。综合疗法的有益作用可能与调节肺表面活性物质蛋白有关。