Ciancio Nicola, Galasso Maria Grazia, Campisi Raffaele, Bivona Laura, Migliore Marcello, Di Maria Giuseppe U
Pneumology Unit, University of Catania, Vittorio Emanuele Hospital, Catania, Italy.
Multidiscip Respir Med. 2012 Sep 14;7(1):29. doi: 10.1186/2049-6958-7-29.
Overexpression of the tumor suppressor gene p53 and the marker for cellular proliferation Ki67 in open lung biopsies are indicated as predictor factors of survival of patients with lung cancer. However, the prognostic value of p53 and Ki67 in fiberoptic bronchial biopsies (FBB) has not been fully investigated. We evaluated p53 and Ki67 immunostaining in FBB from 19 with Non Small-Cell Lung Cancer (NSCLC: 12 adenocarcinomas, 5 squamous cell carcinomas and 2 NSCLC-NOS).
FBB specimens were fixed in formalin, embedded in paraffin, and immunostained using anti-p53 and anti-Ki67 antibodies. Slides were reviewed by two independent observers and classified as positive (+ve) when the number of cells with stained nuclei exceeded 15% for p53 or when >25% positive cells were observed throughout each section for Ki67.
Positive (+ve) immunostaining was found in 9 patients for p53 (47.37%) and 8 patients for Ki67 (42.10%). We examined overall survival curves of the patients with Mantel's logrank test, both p53 -ve and Ki67 -ve patients had significantly higher survival rates than p53 + ve (p < 0.005) and Ki67 + ve (p < 0,0001), respectively.
This study suggests that negative immunostaining of fiberoptic bronchial biopsies for p53 and Ki67 could represent a better prognostic factor for patients with NSCLC.
在开放性肺活检中，肿瘤抑制基因p53的过表达和细胞增殖标志物Ki67被认为是肺癌患者生存的预测因素。然而，p53和Ki67在纤维支气管活检（FBB）中的预后价值尚未得到充分研究。我们评估了19例非小细胞肺癌（NSCLC：12例腺癌、5例鳞状细胞癌和2例NSCLC-NOS）患者FBB中的p53和Ki67免疫染色情况。
FBB标本用福尔马林固定，石蜡包埋，并用抗p53和抗Ki67抗体进行免疫染色。玻片由两名独立观察者复查，当p53染色细胞核的细胞数超过15%或Ki67在每个切片中观察到>25%阳性细胞时，分类为阳性（+ve）。
p53免疫染色阳性（+ve）的患者有9例（47.37%），Ki67免疫染色阳性的患者有8例（42.10%）。我们用Mantel对数秩检验检查了患者的总生存曲线，p53阴性和Ki67阴性的患者生存率分别显著高于p53阳性（p<0.005）和Ki67阳性（p<0.0001）的患者。
本研究表明，纤维支气管活检中p53和Ki67免疫染色阴性可能是NSCLC患者更好的预后因素。
