Maddau Cristina, Confortini Massimo, Bisanzi Simonetta, Janni Aberto, Montinaro Flavio, Paci Eugenio, Pontenani Giovanni, Rulli Pasquale, Salani Anna, Zappa Marco, Benvenuti Alessandra, Carozzi Francesca Maria
Cytopathology Unit, Center for Study and Cancer Prevention, Florence, Italy.
Am J Clin Pathol. 2006 Mar;125(3):425-31.
The purpose of this study was to determine the prognostic significance of the expression of p53 and Ki-67 in non-small cell lung cancer (NSCLC) using immunocytochemical detection. All consecutive NSCLC cases were selected for study, and, after surgery, a part of each tumor sample was frozen at -20 degrees C and stored for immunocytochemical studies. Overexpression of p53 was associated significantly with worse patient outcome in stage I disease, whereas no excess risk was evident in stage II and III cases. The same pattern was observed for Ki-67 expression. The excess risk in stage I cases with p53 and Ki-67 overexpression was observed only in adenocarcinoma. These findings are in agreement with other retrospective studies and support the hypothesis that p53 alteration may have different roles in adenocarcinoma and in squamous cell carcinoma, such as a carcinogenic factor for both cellular types but progression only for adenocarcinoma.
本研究的目的是通过免疫细胞化学检测确定p53和Ki-67表达在非小细胞肺癌（NSCLC）中的预后意义。选取所有连续的NSCLC病例进行研究，术后将每个肿瘤样本的一部分在-20℃冷冻保存用于免疫细胞化学研究。p53过表达与I期疾病患者的较差预后显著相关，而在II期和III期病例中未发现明显的额外风险。Ki-67表达也观察到相同模式。p53和Ki-67过表达的I期病例中的额外风险仅在腺癌中观察到。这些发现与其他回顾性研究一致，并支持以下假设：p53改变在腺癌和鳞状细胞癌中可能具有不同作用，例如对两种细胞类型都是致癌因素，但仅对腺癌有进展作用。
