Jabs C M, Neglen P, Eklof B, Thomas E J
Department of Surgery, Faculty of Medicine, Kuwait University, Safat.
Clin Chem. 1990 Jan;36(1):81-7.
This simple method for sequentially quantifying hypoxanthine (HYP), inosine (INO), and adenosine (ADN) concentrations exploits the H2O2 peroxidase-catalyzed chemiluminescence of luminol. Though applied here only to tissue and plasma, this method can be adapted to analyze other body fluids. HYP in human plasma was stable for 30 min in 10 mmol/L EDTA reagent, whereas ADN was slowly converted to INO. Analytical recovery of HYP and INO added to plasma was 102% each; that of ADN was 95%. The within-run mean CVs for determinations of HYP, INO, and ADN at 1 mumol/L were 3.46%, 2.65%, and 3.01%; at 10 mumol/L they were 2.16%, 1.88%, and 1.63%, respectively. Corresponding between-run CVs were 5.34%, 4.09%, and 4.17%; and 3.43%, 2.40%, and 2.88%, respectively. Bilirubin at a concentration greater than 50 mumol/L interferes, but this interference is eliminated by bilirubin oxidase. Results for both tissue and plasma are compared with previously published results based on different analytical methods.
这种依次定量测定次黄嘌呤（HYP）、肌苷（INO）和腺苷（ADN）浓度的简单方法利用了鲁米诺在过氧化氢过氧化物酶催化下的化学发光现象。尽管此方法目前仅应用于组织和血浆，但它可适用于分析其他体液。人血浆中的HYP在10 mmol/L乙二胺四乙酸（EDTA）试剂中30分钟内保持稳定，而ADN则缓慢转化为INO。添加到血浆中的HYP和INO的分析回收率均为102%；ADN的回收率为95%。在1 μmol/L时，HYP、INO和ADN测定的批内平均变异系数（CV）分别为3.46%、2.65%和3.01%；在10 μmol/L时，分别为2.16%、1.88%和1.63%。相应的批间CV分别为5.34%、4.09%和4.17%；以及3.43%、2.40%和2.88%。浓度大于50 μmol/L的胆红素会产生干扰，但胆红素氧化酶可消除这种干扰。将组织和血浆的结果与先前基于不同分析方法发表的结果进行了比较。
