用高效液相色谱法测定大鼠脑脊液中腺苷、肌苷、次黄嘌呤、黄嘌呤和尿酸的浓度。
Determination of rat cerebrospinal fluid concentrations of adenosine, inosine, hypoxanthine, xanthine and uric acid by high performance liquid chromatography.
作者信息
Walter G A, Phillis J W, O'Reagan M H
机构信息
Department of Physiology, Wayne State University, School of Medicine, Detroit, Michigan 48201.
出版信息
J Pharm Pharmacol. 1988 Feb;40(2):140-2. doi: 10.1111/j.2042-7158.1988.tb05201.x.
Isocratic reverse-phase high performance liquid chromatography techniques were developed to resolve and quantitate the purine nucleosides adenosine (Ado) and inosine (Ino) and their metabolites hypoxanthine (Hyp), xanthine (Xan), and uric acid (UA) in the cerebrospinal fluid of the rat. The moving phase composition for resolving hypoxanthine, xanthine and uric acid was a 0.22 M, pH 5.8 phosphate buffer. The moving phase composition for resolving adenosine and inosine was a 0.22 M, pH 6.8 phosphate buffer, 7% methanol (v/v) and 2.5 mM tetrabutylammonium phosphate. The observed cerebrospinal fluid concentrations in the rat were: Ado = 35 +/- 9 nM (s.e.m.), Ino = 359 +/- 85 nM, Hyp = 243 +/- 77 nM, Xan = 1340 +/- 423 nM and UA = 6130 +/- 678 nM.
开发了等度反相高效液相色谱技术，用于分离和定量大鼠脑脊液中的嘌呤核苷腺苷（Ado）和肌苷（Ino）及其代谢产物次黄嘌呤（Hyp）、黄嘌呤（Xan）和尿酸（UA）。用于分离次黄嘌呤、黄嘌呤和尿酸的流动相组成为0.22 M、pH 5.8的磷酸盐缓冲液。用于分离腺苷和肌苷的流动相组成为0.22 M、pH 6.8的磷酸盐缓冲液、7%甲醇（v/v）和2.5 mM磷酸四丁铵。在大鼠中观察到的脑脊液浓度为：Ado = 35 +/- 9 nM（标准误），Ino = 359 +/- 85 nM，Hyp = 243 +/- 77 nM，Xan = 1340 +/- 423 nM，UA = 6130 +/- 678 nM。
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