Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences, Faculty of Veterinary Medicine and Animal Health, Uppsala, Sweden.
Theriogenology. 2012 Dec;78(9):1901-6. doi: 10.1016/j.theriogenology.2012.06.027. Epub 2012 Sep 12.
It is generally recommended that female cats not intended for planned breeding are spayed to reduce the population of feral cats and also because spaying is beneficial for the long-term health of the individual. For female cats of unknown origin or with estrous symptoms after spaying there is a need for a reliable method to diagnose or rule out the presence of ovaries to avoid unnecessary surgery. Methods previously recommended include vaginal cytology, evaluation of serum estradiol concentration during suspected estrus, induction of ovulation and subsequent evaluation of progesterone, or explorative laparotomy. These methods have the disadvantages that an accurate diagnosis only can be made during estrus or that an invasive procedure is required. Previously, the use of a GnRH challenge test and a semiquantitative LH test have been reported. Our aim was to compare these two methods. We therefore divided 31 female cats in two groups: (1) intact nonestrous females (N = 16), and (2) previously ovariohysterectomized females (N = 15). A blood sample was collected (Time 0) and 0.4 μg/kg buserelin (Receptal; Intervet, Danderyd, Sweden) was injected im. A new blood sample was collected 120 min after the injection. A drop of serum from the sample collected at Time 0 was placed on the LH test (Witness LH; Synbiotics, Corp., San Diego, CA, USA) and the result was evaluated as negative or positive. The remaining serum was frozen and analyzed for estradiol in one batch. Serum estradiol before buserelin stimulation ranged between 5 and 45 pmol/L (N = 14) in intact nonestrous queens and between 2 and 6 pmol/L (N = 15) in ovariohysterectomized females. Estradiol in samples collected after 120 min ranged between 12 and 51 pmol/L (N = 16) in intact queens and between 1 and 7 pmol/L (N = 15) in spayed cats giving a sensitivity and specificity of 100% for the buserelin stimulation test at a cutoff value of 11 pmol/L. All intact queens were negative in the semiquantitative LH test while 14/15 spayed cats were positive and one was negative giving a sensitivity of 100% and a specificity of 93.8% to detect the presence of ovaries in nonestrous cats.
一般建议对不打算繁殖的雌性猫咪进行绝育，以减少流浪猫的数量，同时绝育也有益于猫咪的长期健康。对于来源不明或绝育后出现发情症状的雌性猫咪，需要有一种可靠的方法来诊断或排除卵巢的存在，以避免不必要的手术。以前推荐的方法包括阴道细胞学检查、在疑似发情期间评估血清雌二醇浓度、诱导排卵和随后评估孕酮，或剖腹探查术。这些方法的缺点是只有在发情期才能做出准确的诊断，或者需要进行有创性操作。以前曾报道过使用 GnRH 挑战试验和半定量 LH 试验。我们的目的是比较这两种方法。因此，我们将 31 只雌性猫咪分为两组：（1）未发情的未绝育雌性猫咪（N = 16），和（2）以前已行卵巢子宫切除术的雌性猫咪（N = 15）。采集血样（时间 0），并肌内注射 0.4μg/kg 布舍瑞林（Receptal；Intervet，Danderyd，瑞典）。注射后 120 分钟采集新的血样。将时间 0 采集的样本中的一滴血清滴在 LH 试验（Witness LH；Synbiotics，Corp.，圣地亚哥，CA，美国）上，并将结果评估为阴性或阳性。剩余的血清在一批中冷冻并分析雌二醇。在未发情的未绝育母猫中，布舍瑞林刺激前的血清雌二醇范围为 5 至 45 pmol/L（N = 14），在已行卵巢子宫切除术的母猫中为 2 至 6 pmol/L（N = 15）。120 分钟后采集的样本中的雌二醇范围为 12 至 51 pmol/L（N = 16）在未绝育母猫中，在已绝育母猫中为 1 至 7 pmol/L（N = 15），在 11 pmol/L 的截断值下，布舍瑞林刺激试验的灵敏度和特异性均为 100%。所有未发情的母猫在半定量 LH 试验中均为阴性，而 14/15 只已绝育的母猫为阳性，1 只为阴性，因此该试验在检测未发情的母猫是否存在卵巢时的灵敏度为 100%，特异性为 93.8%。
