Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Theriogenology. 2012 Oct 15;78(7):1627-32. doi: 10.1016/j.theriogenology.2012.07.008.
The rapid growth in sika deer (Cervus nippon) farming and interest in their conservation is an impetus for development of embryo transfer (ET) procedures. However, a paucity of research has prevented widespread application of ET in this species. The objective of the present study was to establish a multiple ovulation and ET procedure with both fresh and vitrified embryos in sika deer. Multiparous weaned hinds (N = 18) were used as embryo donors during the reproductive season of 2008 at a local breeding farm in China. Estrus was synchronized in donors and recipients (N = 38) by inserting a controlled internal drug release for 12 days (insertion = Day 0). Superovulation was induced with a total of 320 mg of NIH-FSH-P1 (Folltropin-V; Bioniche, Belleville, ON, Canada) given as 40 mg im every 12 h from the afternoon of Day 9 to the morning of Day 13. After estrus was detected, donors were artificially inseminated using a transcervical technique. The embryo recovery rate was 76.8% (63/82), including 1.6% (1/63), 77.8% (49/63), and 1.6% (1/63) blastocysts, morula, and eight-cell embryos, respectively. After transfer of fresh and vitrified embryos, pregnancy rates were 85.7% and 61.6% and birth rates were 64.3% and 53.9% (P > 0.05). In conclusion, we developed a satisfactory multiple ovulation and ET procedure in farmed sika deer using vitrified embryos.
圈养梅花鹿（ Cervus nippon ）养殖的快速增长以及对其保护的关注，是胚胎移植（ ET ）技术发展的动力。然而，由于缺乏研究，该技术在该物种中的广泛应用受到了阻碍。本研究的目的是建立一种梅花鹿新鲜胚胎和冷冻胚胎的多次排卵和 ET 技术。 2008 年繁殖季节，在当地的一个养殖场，使用已断奶的多胎经产母鹿（ N = 18 ）作为胚胎供体。供体和受体（ N = 38 ）通过插入 12 天的控释宫内节育器来同步发情（插入 = 第 0 天）。从第 9 天下午到第 13 天上午，每隔 12 小时肌内注射总共 320 mg NIH-FSH-P1 （ Folltropin-V ； Bioniche ， Belleville ， ON ，加拿大），诱导超数排卵。发情后，通过经宫颈技术对供体进行人工授精。胚胎回收率为 76.8%（ 63/82 ），包括 1.6%（ 1/63 ）、 77.8%（ 49/63 ）、 1.6%（ 1/63 ）的囊胚、桑椹胚和 8 细胞胚胎。新鲜胚胎和冷冻胚胎移植后的妊娠率分别为 85.7%和 61.6%，出生率分别为 64.3%和 53.9%（ P > 0.05 ）。总之，我们使用冷冻胚胎在圈养梅花鹿中建立了一种满意的多次排卵和 ET 技术。
