Soler J P, Mucci N, Kaiser G G, Aller J, Hunter J W, Dixon T E, Alberio R H
Actividad Privada, Argentina.
Anim Reprod Sci. 2007 Dec;102(3-4):322-7. doi: 10.1016/j.anireprosci.2007.03.024. Epub 2007 Mar 30.
Two multiple ovulation and embryo transfer (MOET) programs with fresh, frozen and vitrified red deer embryos were carried out during the reproductive season of 2005 and 2006 in a local breeding farm in Argentina. Multiparous (n=10 and 9, respectively) weaned hinds were used as donors for each year. The estrous synchronization treatment of donors and recipients consisted of inserting an ovine intravaginal sponge containing medroxiprogesterone acetate (MAP) for 12 days. Superovulation was conducted with a total dose of 180 mg of NIH-FSH-P1 (Folltropin-V, Bioniche, Belleville, Ontario, Canada), given i.m. in eight decreasing doses every 12h (40, 40; 27, 27; 15, 15; 8, 8 mg), from days 10 to 13. Donor females were mated with one stag of proven fertility. The recovery rate was 84.1% (122/145), obtaining 45.1% (55/122) of transferable embryos, 24.6% (30/122) of degenerated embryos and 30.3% (37/122) of unfertilized oocytes. Pregnancy rates after transfer of fresh, OPS vitrified/warmed and ethylene glycol (EG) frozen/thawed embryos were 64.3% (18/28), 53.3% (8/15) and 70.0% (7/10), respectively. Vitrification and freezing with ethylene glycol procedures constitute an interesting alternative for red deer embryo cryopreservation.
2005年和2006年繁殖季节期间，在阿根廷当地一个养殖场开展了两个使用新鲜、冷冻和玻璃化马鹿胚胎的多次排卵和胚胎移植（MOET）项目。每年分别选用经产（分别为n = 10头和9头）断奶母鹿作为供体。供体和受体的发情同步处理是插入含醋酸甲羟孕酮（MAP）的绵羊阴道海绵栓12天。从第10天到第13天，通过肌肉注射总剂量为180 mg的美国国立卫生研究院促卵泡素-P1（Folltropin-V，Bioniche，加拿大安大略省贝尔维尔）进行超排卵，每12小时分8次递减剂量给药（40、40；27、27；15、15；8、8 mg）。供体母鹿与一头经证实有生育能力的雄鹿交配。回收率为84.1%（122/145），可移植胚胎占45.1%（55/122），退化胚胎占24.6%（30/122），未受精卵母细胞占30.3%（37/122）。新鲜胚胎、OPS玻璃化/复温胚胎和乙二醇（EG）冷冻/解冻胚胎移植后的妊娠率分别为64.3%（18/28）、53.3%（8/15）和70.0%（7/10）。玻璃化和乙二醇冷冻程序是马鹿胚胎冷冻保存的一个有趣替代方法。
