Department of Pharmaceutical Analysis & Metabolomics and Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Province Academy of Traditional Chinese Medicine, No. 100 Shizi Street, Hongshan Road, Nanjing 210028, PR China.
Food Chem. 2012 Dec 15;135(4):2277-86. doi: 10.1016/j.foodchem.2012.07.006. Epub 2012 Jul 13.
In this study, a sensitive and selective method for simultaneously quantifying eight major components (four iridoid glycosides, three phenethylalcohol glycosides and one furfural derivative) of Rehmanniae Radix by high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (HPLC-TQ-MS) was developed. The sample preparation was executed using an optimised ultrasonic method with complete extraction efficiencies of eight analytes. For mass spectrometry, selected ion recording (SIR) scan mode was used to improve the sensitivity and selectivity. The established method was validated in terms of linearity, sensitivity, precision, accuracy and stability, and successfully applied to determine the contents of the eight analytes in different batches of raw and processed Rehmanniae Radix, which confirmed that the established method was reliable and useful for "holistic" quality evaluation of Rehmanniae Radix. The quantitative results indicated that the quality of commercial raw or processed Rehmanniae Radix was remarkably inconsistent.
本研究建立了一种同时测定地黄中 8 种主要成分（4 种环烯醚萜苷、3 种苯乙醇苷和 1 种糠醛衍生物）的高效液相色谱-串联三重四极杆质谱（HPLC-TQ-MS）分析方法。样品制备采用优化的超声提取方法，8 种分析物的提取效率完全。质谱采用选择离子记录（SIR）扫描模式，以提高灵敏度和选择性。该方法经过线性、灵敏度、精密度、准确度和稳定性验证，成功应用于不同批次生地黄和熟地黄中 8 种成分的含量测定，表明该方法可靠、实用，可用于地黄的“整体”质量评价。定量结果表明，市售生地黄或熟地黄的质量差异显著。
