炮制地黄提取物对血虚大鼠模型影响的代谢组学策略及生化分析。
Metabolomic strategies and biochemical analysis of the effect of processed Rehmanniae radix extract on a blood-deficient rat model.
机构信息
College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China.
The Engineering and Technology Center for Chinese Medicine Development of Henan Province, 156 Jinshui East Road, Zhengzhou, 450046, China.
出版信息
BMC Complement Med Ther. 2022 Mar 25;22(1):89. doi: 10.1186/s12906-022-03560-x.
BACKGROUND
Rehmanniae Radix (RR), an herb with numerous pharmacological effects, is widely used in traditional Chinese medicine for the treatment of blood deficiency syndrome, either alone or in combination with other herbs. However, the mechanism by which processed Rehmanniae Radix (PRR) improves blood enrichment efficacy has not been clearly defined.
METHODS
Ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass (UPLC-Q-TOF/MS) and biochemical methods were combined to explore the hematopoietic functional mechanisms of PRR on blood deficiency in a rat model, as well as the potential active ingredient for blood enrichment efficacy. The pharmacological effects of PRR were evaluated on a rat blood deficiency model induced by cyclophosphamide in combination with 1-acetyl-2-phenylhydrazine. The blood routine index, including white blood cell (WBC), red blood cell (RBC), and platelet (PLT) counts, as well as hemoglobin (HGB) level, and the changing metabolite profile based on urine and serum were assessed. Nontargeted metabolomic studies, combined with biochemical analyses, were employed to clarify pharmacological mechanisms.
RESULTS
PRR significantly increased the blood routine index levels and reversed the levels of SOD, GSH, and ATP. The PRR group was similar to the control group, as determined from the metabolic profile. All of the 60 biomarkers, representing the typical metabolic characteristics of the blood-deficient rat model, mainly involved energy metabolism dysfunction, the peripheral circulation system, and oxidative damage in the body. This improvement may be attributed to changes in polysaccharide and sixteen non-polysaccharide compounds in PRR, which were caused by processing RR with rice wine.
CONCLUSIONS
The strategies of integrated metabolomic and biochemical analyses were combined, revealing the biological function and effective mechanism of PRR.
背景
地黄（RR）是一种具有多种药理作用的草药，在传统中药中广泛用于治疗血虚症，单独使用或与其他草药联合使用。然而，炮制地黄（PRR）提高补血功效的机制尚未明确。
方法
采用超高效液相色谱-四极杆飞行时间质谱联用（UPLC-Q-TOF/MS）和生化方法相结合，探讨 PRR 对血虚大鼠模型的造血功能机制及潜在的补血功效活性成分。以环磷酰胺联合 1-乙酰-2-苯肼诱导大鼠血虚模型评价 PRR 的药理作用。检测血常规指标，包括白细胞（WBC）、红细胞（RBC）和血小板（PLT）计数以及血红蛋白（HGB）水平，以及基于尿液和血清的代谢物谱变化。采用非靶向代谢组学研究结合生化分析阐明药理机制。
结果
PRR 显著提高了血常规指标水平，逆转了 SOD、GSH 和 ATP 的水平。PRR 组与对照组相似，从代谢谱可以看出。60 个生物标志物代表了血虚大鼠模型的典型代谢特征，主要涉及能量代谢功能障碍、外周循环系统和体内氧化损伤。这种改善可能归因于 PRR 中多糖和十六种非多糖化合物的变化，这是由地黄用黄酒炮制引起的。
结论
整合代谢组学和生化分析的策略相结合，揭示了 PRR 的生物学功能和有效机制。
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