Key Laboratory of Poyang Lake Ecology and Bio-Resource Utilization of Ministry of Education, Nanchang University, China.
Food Chem. 2012 Dec 15;135(4):2299-306. doi: 10.1016/j.foodchem.2012.07.038. Epub 2012 Jul 15.
Ionic liquid (IL) based ultrasonic assisted extraction (ILUAE) was developed for the effective extraction of puerarin from Radix Puerariae Lobatae (RPL). The ILUAE parameters including the type of ILs, IL concentration, RPL amount, ultrasonic power and time were optimized by single-factor experiment and response surface methodology. Under the optimized experimental conditions, the best results were obtained using RPL amount 0.43 g in 10 mL 1.06 molL(-1) 1-butyl-3-methylimidazolium bromide aqueous solution, ultrasonic time 27.43 min and ultrasonic power 480 W. Scanning electron microscope images of RPL samples were obtained to provide visual evidence of the sonication effect. Compared with the conventional ultrasonic assisted extraction and refluent extraction, the proposed ILUAE offered shorter extraction time and remarkable higher efficiencies due to the higher penetration ability and solubility of IL and the cavitation phenomenon produced in the solvent by the passage of an ultrasonic wave, which further supported the suitability of the proposed approach.
离子液体（IL）辅助超声提取（ILUAE）被开发用于从葛根（RPL）中有效提取葛根素。通过单因素实验和响应面法优化了 ILUAE 参数，包括 IL 类型、IL 浓度、RPL 量、超声功率和时间。在优化的实验条件下，在 0.43 g RPL 量下，使用 10 mL 1.06 molL(-1) 1-丁基-3-甲基咪唑溴水溶液、27.43 分钟超声时间和 480 W 超声功率，可获得最佳结果。对 RPL 样品进行扫描电子显微镜图像分析，为超声效应提供了直观证据。与传统的超声辅助提取和回流提取相比，由于 IL 具有更高的穿透能力和溶解度，以及超声波在溶剂中产生的空化现象，提出的 ILUAE 具有更短的提取时间和显著更高的效率，进一步支持了该方法的适用性。
