Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China.
Food Chem. 2013 Dec 1;141(3):2426-33. doi: 10.1016/j.foodchem.2013.05.094. Epub 2013 May 25.
An ionic liquids-based ultrasonic-assisted extraction (ILUAE) method was successfully developed for extracting eleutheroside B and E from Radix Acanthopanax senticosus. Thirteen 1-alkyl-3-methylimidazolium ionic liquids with different cations and anions were investigated and 1-butyl-3-methylimidazolium bromide ([C4mim]Br) solution was selected as the solvent. The conditions for ILUAE, including the ionic liquid concentration, soaking time, ultrasonic power, ultrasonic time, solid-liquid ratio and number of extraction cycles, were optimized. With the proposed method, the energy consumption time was reduced to 30 min, whereas conventional method requires about 4h. The proposed method had good recovery (97.96-103.39%) and reproducibility (RSD, n=5; 3.3% for eleutheroside B, 4.6% for eleutheroside E). ILUAE was an efficient, rapid and simple sample preparation technique that showed high reproducibility and was environmental friendly.
一种基于离子液体的超声辅助提取（ILUAE）方法被成功开发用于从刺五加中提取刺五加苷 B 和 E。研究了 13 种具有不同阳离子和阴离子的 1-烷基-3-甲基咪唑离子液体，并选择 1-丁基-3-甲基咪唑溴化物（[C4mim]Br）溶液作为溶剂。优化了 ILUAE 的条件，包括离子液体浓度、浸泡时间、超声功率、超声时间、固液比和提取循环次数。与传统方法相比，该方法的能耗时间从 4 小时缩短至 30 分钟。该方法的回收率良好（97.96-103.39%），重现性好（RSD，n=5；刺五加苷 B 的 RSD 为 3.3%，刺五加苷 E 的 RSD 为 4.6%）。ILUAE 是一种高效、快速、简单的样品制备技术，具有高重现性且环保。
