State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Biomaterials. 2012 Dec;33(34):8802-11. doi: 10.1016/j.biomaterials.2012.08.054. Epub 2012 Sep 12.
Improving vascularization of tissue-engineered bone can advance cell performance in vivo and further promote bone regeneration. How to achieve a functional vascular network within the construct is one of the biggest challenges so far. We hypothesized that a mixture of platelet-rich plasma (PRP) and adipose-derived stem cells (ADSCs) could endue the alginate microspheres with osteogenic and angiogenic potential. In vitro and in vivo studies were performed to investigate the potential of the PRP-ADSC-laden microspheres. Two intriguing observations were made in this study. First, we demonstrated that PRP sustained cell viability and meanwhile promoted cell migration from the interior of alginate microspheres to the surface. This phenomenon indicated that encapsulated cells have the potential to directly and actively participate into the regeneration process. Second, in vivo, a blood vessel network was found within the 10% PRP and 15% PRP-ADSC implants, which was associated with a significant increase in mineralization. It suggested that the PRP-ADSC-laden microspheres did enhance the vascularization and mineralization. In summary, this strategy not only provides a micro-invasive therapy for bone regeneration, but also could be incorporated with other matrices for extended application.
改善组织工程骨的血管化可以促进细胞在体内的性能，并进一步促进骨再生。如何在构建体中实现功能性血管网络是迄今为止最大的挑战之一。我们假设富含血小板的血浆（PRP）和脂肪来源的干细胞（ADSCs）的混合物可以使藻酸盐微球具有成骨和血管生成潜力。进行了体外和体内研究来研究载有 PRP-ADSC 的微球的潜力。本研究中有两个有趣的观察结果。首先，我们证明 PRP 维持细胞活力，同时促进细胞从藻酸盐微球的内部迁移到表面。这一现象表明，被包裹的细胞有可能直接和主动参与到再生过程中。其次，在体内，在 10% PRP 和 15% PRP-ADSC 植入物中发现了血管网络，这与矿化的显著增加有关。这表明载有 PRP-ADSC 的微球确实增强了血管生成和矿化。总之，这种策略不仅为骨再生提供了微创治疗，而且还可以与其他基质结合以进行扩展应用。
