Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, China.
Tissue Eng Part A. 2011 Mar;17(5-6):725-39. doi: 10.1089/ten.TEA.2010.0331. Epub 2010 Dec 18.
Slow vascularization rate is considered one of the main drawbacks of scaffolds used in wound healing. Several efforts, including cellular and acellular technologies, have been made to induce vascular growth in scaffolds. However, thus far, there is no established technology for inducing vascular growth. The aim of this study was to promote the vascularization capacities of scaffolds by seeding adipose-derived stem cells (ADSCs) on them and to compare the vascularization capacities of different scaffolds seeded with ADSCs. Two kinds of extracellular matrix scaffolds (small intestinal submucosa [SIS] and acellular dermal matrix [ADM]) and a kind of composite scaffold (collagen-chondroitin sulfate-hyaluronic acid [Co-CS-HA]) were selected. Subcutaneous implantation analysis showed that the vascularization capacity of SIS and ADM was greater than that of Co-CS-HA. ADSCs seeded in SIS and ADM secreted greater amounts of vascular endothelial growth factor than those seeded in Co-CS-HA. In a murine skin injury model, ADSC-seeded scaffolds enhanced the angiogenesis and wound healing rate compared with the nonseeded scaffolds. Moreover, ADSC-SIS and ADSC-ADM had greater vascularization capacity than that of ADSC-Co-CS-HA. Taken together, these results suggest that ADSCs could be used as a cell source to promote the vascularization capacities of scaffolds. The vascularization capacities of ADSC-seeded scaffolds were influenced by both the vascularization capacities of the scaffolds themselves and their effects on the angiogenic potential of ADSCs; the combination of extracellular matrix scaffolds and ADSCs exhibited synergistic angiogenesis promoting effects.
血管化速度缓慢被认为是用于伤口愈合的支架的主要缺点之一。已经做出了包括细胞和去细胞技术在内的几种努力来诱导支架中的血管生长。然而，到目前为止，还没有用于诱导血管生长的既定技术。本研究的目的是通过在支架上种植脂肪来源的干细胞（ADSCs）来促进支架的血管化能力，并比较不同支架上种植 ADSCs 的血管化能力。选择了两种细胞外基质支架（小肠黏膜下层[SIS]和去细胞真皮基质[ADM]）和一种复合支架（胶原-硫酸软骨素-透明质酸[Co-CS-HA]）。皮下植入分析表明，SIS 和 ADM 的血管化能力大于 Co-CS-HA。在 SIS 和 ADM 中种植的 ADSCs 分泌的血管内皮生长因子多于在 Co-CS-HA 中种植的 ADSCs。在小鼠皮肤损伤模型中，与未种植支架相比，ADSC 种植的支架增强了血管生成和伤口愈合速度。此外，ADSC-SIS 和 ADSC-ADM 的血管化能力大于 ADSC-Co-CS-HA。综上所述，这些结果表明 ADSCs 可用作促进支架血管化能力的细胞来源。ADSC 种植支架的血管化能力受到支架自身血管化能力及其对 ADSCs 血管生成潜力的影响；细胞外基质支架和 ADSCs 的结合表现出协同促进血管生成的作用。
