Clô C, Caldarera C M, Tantini B, Benalal D, Bachrach U
Biochem J. 1979 Sep 15;182(3):641-9. doi: 10.1042/bj1820641a.
The effect of polyamines on the cellular concentrations of cyclic AMP was studied. It was shown that 1 microM-spermine caused a decrease in cyclic AMP in chick-embryo heart cells, chick-embryo fibroblasts, neuroblastoma, glioma and neuroblastoma-glioma hybrid cells, grown in culture. A similar decrease was observed when polyamines were added to cells in the presence of a phosphodiesterase inhibitor or after stimulating the cells with various hormones. Noradrenaline was used in cultures of heart cells, prostaglandin E1 and adenosine for neuroblastoma and neuroblastoma-glioma hybrids, whereas isoproterenol was used for the stimulation of glioma cells. Polyamines at higher concentrations were either without effect or caused a slight increase in cyclic AMP. Spermidine (10 microM) also caused a decrease in cellular cyclic AMP, as did 0.1 microM-putrescine. It is suggested that the effect of polyamines on cellular cyclic AMP may be explained by the effect of these polycations on the activity of cellular phosphodiesterase.
研究了多胺对细胞内环磷酸腺苷(cAMP)浓度的影响。结果表明,在培养的鸡胚心脏细胞、鸡胚成纤维细胞、神经母细胞瘤、胶质瘤以及神经母细胞瘤 - 胶质瘤杂交细胞中,1微摩尔精胺会导致cAMP减少。当在磷酸二酯酶抑制剂存在的情况下向细胞中添加多胺,或用各种激素刺激细胞后,也观察到了类似的减少。在心脏细胞培养中使用去甲肾上腺素,在神经母细胞瘤和神经母细胞瘤 - 胶质瘤杂交细胞培养中使用前列腺素E1和腺苷,而在胶质瘤细胞刺激中使用异丙肾上腺素。较高浓度的多胺要么没有效果,要么会导致cAMP略有增加。亚精胺(10微摩尔)也会导致细胞内cAMP减少,0.1微摩尔腐胺也有同样的效果。有人提出,多胺对细胞内cAMP的影响可能是由这些多阳离子对细胞磷酸二酯酶活性的影响来解释的。