Kram R, Mamont P, Tomkins G M
Proc Natl Acad Sci U S A. 1973 May;70(5):1432-6. doi: 10.1073/pnas.70.5.1432.
The effects of serum deprivation on several general cellular biochemical processes ("pleiotypic response") related to the growth of normal fibroblasts can be mimicked by treatment of these cells with prostaglandin E(1) in the presence of serum. N(6),O(2)'-Dibutyryl adenosine 3':5'-cyclic monophosphate and theophylline inhibit the membrane transport processes without much effect on other pleiotypic reactions such as overall protein and RNA synthesis and protein degradation. The amount of intracellular cyclic AMP increases during serum starvation and returns to the initial concentration in unstarved cells when growth is initiated again upon addition of serum. Fibroblasts transformed by simian virus 40 have a lower cyclic AMP content than their untransformed parents. Serum deprivation neither increases cyclic AMP content nor significantly affects the pleiotypic reactions in transformed cells. Cycloheximide causes a decrease in cyclic AMP content of normal fibroblasts coincidentally with the ability of this inhibitor to stimulate uridine transport and slow protein degradation in cells deprived of serum.
血清剥夺对与正常成纤维细胞生长相关的几种一般细胞生化过程（“多型性反应”）的影响，可以通过在有血清存在的情况下用前列腺素E(1)处理这些细胞来模拟。N(6),O(2)'-二丁酰腺苷3':5'-环一磷酸和茶碱抑制膜转运过程，而对其他多型性反应如总蛋白质和RNA合成以及蛋白质降解影响不大。在血清饥饿期间，细胞内环磷酸腺苷的量增加，当再次添加血清开始生长时，其会恢复到未饥饿细胞的初始浓度。被猿猴病毒40转化的成纤维细胞的环磷酸腺苷含量低于未转化的亲本细胞。血清剥夺既不会增加环磷酸腺苷含量，也不会显著影响转化细胞中的多型性反应。环己酰亚胺会导致正常成纤维细胞的环磷酸腺苷含量降低，与此同时，这种抑制剂能够刺激尿苷转运并减缓血清剥夺细胞中的蛋白质降解。
