RNA聚合酶II对启动子的清除。
Promoter clearance by RNA polymerase II.
作者信息
Luse Donal S
机构信息
Department of Molecular Genetics, Cleveland Clinic, Cleveland, OH 44195, USA.
出版信息
Biochim Biophys Acta. 2013 Jan;1829(1):63-8. doi: 10.1016/j.bbagrm.2012.08.010. Epub 2012 Sep 6.
Abstract
Many changes must occur to the RNA polymerase II (pol II) transcription complex as it makes the transition from initiation into transcript elongation. During this intermediate phase of transcription, contact with initiation factors is lost and stable association with the nascent transcript is established. These changes collectively comprise promoter clearance. Once the transcript elongation complex has reached a point where its properties are indistinguishable from those of complexes with much longer transcripts, promoter clearance is complete. The clearance process for pol II consists of a number of steps and it extends for a surprisingly long distance downstream of transcription start. This article is part of a Special Issue entitled: RNA polymerase II Transcript Elongation.
摘要
当RNA聚合酶II（pol II）转录复合物从起始阶段过渡到转录延伸阶段时，会发生许多变化。在转录的这个中间阶段，与起始因子的接触丧失，与新生转录本建立稳定关联。这些变化共同构成启动子清除。一旦转录延伸复合物达到其特性与具有长得多的转录本的复合物无法区分的程度，启动子清除就完成了。pol II的清除过程由多个步骤组成，并且在转录起始点下游延伸出惊人的长距离。本文是名为：RNA聚合酶II转录延伸的特刊的一部分。
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1
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Nucleic Acids Res. 2012 Aug;40(14):6495-507. doi: 10.1093/nar/gks323. Epub 2012 Apr 17.
2
Genome-wide structure and organization of eukaryotic pre-initiation complexes.真核生物起始前复合物的全基因组结构和组织。
Nature. 2012 Jan 18;483(7389):295-301. doi: 10.1038/nature10799.
3
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Mol Cell. 2012 Jan 13;45(1):38-50. doi: 10.1016/j.molcel.2011.10.022.
4
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J Biol Chem. 2012 Jan 6;287(2):961-7. doi: 10.1074/jbc.M111.297572. Epub 2011 Nov 27.
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