RNA聚合酶II转录：结构与机制
RNA polymerase II transcription: structure and mechanism.
作者信息
Liu Xin, Bushnell David A, Kornberg Roger D
机构信息
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
出版信息
Biochim Biophys Acta. 2013 Jan;1829(1):2-8. doi: 10.1016/j.bbagrm.2012.09.003. Epub 2012 Sep 18.
Abstract
A minimal RNA polymerase II (pol II) transcription system comprises the polymerase and five general transcription factors (GTFs) TFIIB, -D, -E, -F, and -H. The addition of Mediator enables a response to regulatory factors. The GTFs are required for promoter recognition and the initiation of transcription. Following initiation, pol II alone is capable of RNA transcript elongation and of proofreading. Structural studies reviewed here reveal roles of GTFs in the initiation process and shed light on the transcription elongation mechanism. This article is part of a Special Issue entitled: RNA Polymerase II Transcript Elongation.
摘要
一个最小的RNA聚合酶II（pol II）转录系统由该聚合酶和五个通用转录因子（GTF）TFIIB、-D、-E、-F及-H组成。添加中介体可实现对调控因子的响应。GTF是启动子识别和转录起始所必需的。起始之后，仅pol II就能进行RNA转录本的延伸和校对。本文综述的结构研究揭示了GTF在起始过程中的作用，并阐明了转录延伸机制。本文是名为“RNA聚合酶II转录延伸”的特刊的一部分。
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